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In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.

Separation of Five Steroids on a Hamilton PRP-C18 Reversed-Phase HPLC Column

Chitosans are analyzed by SEC-MALS to determine molar mass moments and distributions. 

Absolute Molar Mass Analysis of Chitosans

The purpose of this study is to demonstrate the use of mass spectrometry to characterize the elution profile of a typical IgG1 molecule separation on a TSKgel FcR-IIIA-NPR column, and verify the observations that certain glycan structures impart higher activity to the monoclonal antibody, especially as it relates to the presence of terminal galactose sugars.

Characterization of TSKgel FcR-IIIA-NPR HPLC Column by Top Down Mass Spectrometry

This application note effectively outlines a simple three-step method for the extraction of loperamide and its main metabolite, N-desmethyl loperamide, from blood. 

Medical/Biological