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On a fundamental level, equilibrium and diffusion define chemical separations. Practical applications of partition coefficients to chromatographic sample preparation illustrate this point.

Practical Understanding of Partition Coefficients

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.

Separation Science: The State of the Art: New Frontiers in Multidimensional Liquid Chromatography

This review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.

Method Development for Reversed-Phase Separations of Peptides: A Rational Screening Strategy for Column and Mobile Phase Combinations with Complementary Selectivity

The methodologies demonstrated here open the way to fully compliant at-line monitoring of monoclonal antibody quality attributes.

New 2D-LC–MS Approaches for the Analysis of In-Process Samples and for the Characterization of mAbs in a Regulated Environment

This article explores potential pitfalls associated with 1D‑LC and how 2D‑LC can overcome these obstacles.

Minimizing the Risk of Missing Critical Sample Information by Using 2D-LC

Having a mixed-mode column that can provide both achiral and chiral resolution in one-dimension (1D) would significantly improve impurity profile understanding and reduce testing workload.

One-Dimensional Simultaneous Achiral and Chiral Analysis

In this article, I share my perspective on the trends in 2D-LC, and the developments we are likely to see in the field in the near future.

The Future of Method Development for Two-Dimensional Liquid Chromatography – Work Smarter, Not Just Harder?

As two-dimensional liquid chromatography (2D-LC) becomes more widely used, system suitability tests (SSTs) become even more important.

Development of a System Suitability Test for Two-Dimensional Liquid Chromatography

This month we interview Alexandre Goyon, Senior Scientist in the Small Molecule Pharmaceutical Sciences Organization of Genentech, about his work focused on the online digestion and analysis of RNA molecules using immobilized RNase cartridges attached to a mDLC–MS system, and why the accurate sequencing of sgRNA is important.

Rising Stars of Separation Science: Alexandre Goyon

Multidimensional liquid chromatography (MDLC) methods have revolutionized the characterization of complex drug modalities like antibodies–drug conjugates, antisense oligonucleotides, and small interfering RNA therapeutics.

Multidimensional LC | Topics