Transferring Methods to Compact and Portable HPLC (February 2024)
February 14th 2024The current trend in laboratory equipment design is the miniaturization of laboratory instruments. Smaller-scale HPLC instruments offer benefits that cannot be matched by analytical-scale equipment, especially in the areas of portability, reduced fluid volumes, and reduced operating costs. Yet, the miniaturization of laboratory equipment has brought with it a unique set of challenges, including transferring methods to compact LC. Capillary LC expands the use of LC to applications not currently done using conventional LC in a wide array of application areas, including pharmaceutical, food and beverage, petrochemical, environmental, and oil and gas. Greg Ward, Axcend’s CEO wrote, “Customers want an HPLC system with a small footprint, low flow rates and green chemistry.” Join his podcast where he shares method transfer in these application areas.
Prioritizing Purity: How Pure is Pure Enough? (December 2023)
December 6th 2023When it comes to high-purity inorganic raw materials, the key to analytical success is knowing how to define purity. A variety of factors can impact this definition, including the specific application as well as the chemist’s needs.
LC Column Hardware Evolution and its Role in Sustainability (September 2023)
September 18th 2023In this episode, “LC Column Hardware Evolution and its Role in Sustainability” brought to you by Advanced Materials Technology, Brett Paull Ph.D., Director of the ARC Training Centre for Hyphenated Analytical Separation technologies (HyTECH) at the University of Tasmania discusses the relationship of HPLC column technology and sustainability at the laboratory level.
Replacing 40+ Year-Old Analytical Techniques (September 2023)
September 8th 2023Pharmaceutical science has used the same slow analytical quality testing techniques for more than 40 years. Dr. Fred Regnier, CTO of Novilytic and one of the world’s top analytical chemists, explains how a new method is being utilized to expedite the molecular analysis of antibodies and to eliminate sample prep and mass spectrometry.
The Future of Chromatography Sample Preparation: Trends in Solid-phase Extraction Methods
July 11th 2023James Edwards, an Application Scientist and Chromatographer at Porvair Sciences, discusses the challenges and advancements in solid-phase extraction (SPE) as a sample preparation technique for chromatography.
Characterizing Polymer Architecture: Importance, Challenges, Solution
April 17th 2023Polymers are gaining more attention in many new fields such as pharmaceutical applications. However, optimizing the final properties of the polymers associated with these new application areas is challenging for chemists and analysts. Polymer architecture plays a crucial role to optimize the end use or process-related properties. Size exclusion chromatography in combination with advanced detectors such as light scattering, viscometer, and refractive index is a powerful tool for the comprehensive characterization of macromolecules with various architectures. In this podcast, Dr. Subin Damodaran will explain how to understand the structural properties of a polymer by providing their molecular sizes such as radius of gyration (Rg ) and hydrodynamic radius (Rh), and intrinsic viscosity.
Stable Isotope Labelled Internal Standard (SIL-IS) for Analysis of Proteins and mAbs with LC-MS
March 10th 2023Quantitative measurement of biotherapeutic and clinically important biomarker proteins using LC-MS/MS has seen great development in the last 15 years. Stable Isotope Labelled Internal Standards are a cornerstone of accurate quantitation and there are several options ranging from signature peptides to “winged” longer peptides or full 13C and/or 15N labelled proteins to choose from. In this video Kevin Ray, Kwasi Mawunyega and Anders Fridstrom will discuss pros and cons with different SIL IS approaches as well as similarities and differences between biopharmaceutical bioanalysis and biomarkers for disease research & diagnosis.
Stable Isotope Labelled Internal Standard (SIL-IS) for analysis of Proteins and mAbs with LC-MS
March 10th 2023Quantitative measurement of biotherapeutic and clinically important biomarker proteins using LC-MS/MS has seen great development in the last 15 years. Stable Isotope Labelled Internal Standards are a cornerstone of accurate quantitation and there are several options ranging from signature peptides to “winged” longer peptides or full 13C and/or 15N labelled proteins to choose from. In this video Kevin Ray, Kwasi Mawunyega and Anders Fridstrom will discuss pros and cons with different SIL IS approaches as well as similarities and differences between biopharmaceutical bioanalysis and biomarkers for disease research & diagnosis.