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Preparative chromatography has been widely used as a purification technique for many years and continues to enjoy growth in the separations market. Joining us for this forum are Frédéric Cheviron of Chiral Technologies Europe, J.T. Presley of Phenomenex and Jean Bléhaut of Novasep.

Technology Forum: Preparative Chromatography

Preparative chromatography has been widely used as a purification technique for many years, particularly in the pharmaceutical industry. Joining us for this forum are Frédéric Cheviron of Chiral Technologies Europe, J.T. Presley of Phenomenex, and Jean Bléhaut of Novasep.

Flash chromatography was first introduced by W.C. Still in 1978. The following report published in the June 2006 issue of LCGC North America provided a good overview of the technique.

Ask the Editor: The Evolution of Flash Chromatography

lthough liquid chromatography (LC) is most commonly associated with analytical techniques that are found inside the laboratory, LC also has been used in industrial applications for separation of a wide range of products for decades. The technique recently has been applied to requirements in the pharmaceutical and biotechnology industries, both inside the laboratory and in industrial settings. With global sales of over $1.7 billion, the market for preparative LC has encountered a stellar growth over the past few years.

Preparative and Process Chromatography

Although liquid chromatography (LC) is most commonly associated with analytical techniques that are found inside the laboratory, LC also has been used in industrial applications for separation of a wide range of products for decades.

HPLC Mar 07

HPLC is among the most dynamic markets in the laboratory analytical and life science instrument industry. The market can be segmented into six different categories, which include conventional HPLC, capillary/nano LC, fast LC, preparative HPLC, amino acid analyzers, and gel permeation chromatography systems. Conventional HPLC systems, which account for the majority of the market, are standard HPLC instruments with typical flow rates between 1–10 mL/min. However, other HPLC systems designed for specific applications and that remedy some of the shortcomings of conventional LC systems, are becoming more significant in the market.

High Performance Liquid Chromatography

Purification of synthetic, natural and biological compounds in any quantity usually requires the use of preparative HPLC.

Factors and Parameters that Directly Affect Recovery of Collected Fractions in Preparative HPLC

This column focuses on some of the latest developments in preparative-scale columns, bulk packing materials and column hardware designs. Silica, polymeric and other packings are discussed together with the newest monolithic columns.

Developments in Preparative-Scale Chromatography Columns and Accessories

Column Editor Ron Majors turns his attention to preparative chromatography. He focuses on the columns used in preparative chromatography, including how to select the appropriate mode, mobile-phase system and operating conditions.