Preparative-Scale Chromatography

HPLC is among the most dynamic markets in the laboratory analytical and life science instrument industry. The market can be segmented into six different categories, which include conventional HPLC, capillary/nano LC, fast LC, preparative HPLC, amino acid analyzers, and gel permeation chromatography systems. Conventional HPLC systems, which account for the majority of the market, are standard HPLC instruments with typical flow rates between 1–10 mL/min. However, other HPLC systems designed for specific applications and that remedy some of the shortcomings of conventional LC systems, are becoming more significant in the market.

Purification of synthetic, natural and biological compounds in any quantity usually requires the use of preparative HPLC.

This column focuses on some of the latest developments in preparative-scale columns, bulk packing materials and column hardware designs. Silica, polymeric and other packings are discussed together with the newest monolithic columns.

Column Editor Ron Majors turns his attention to preparative chromatography. He focuses on the columns used in preparative chromatography, including how to select the appropriate mode, mobile-phase system and operating conditions.

A look at the latest developments in preparative-scale columns, bulk packing materials, and column hardware designs.

This article describes the optimum performance laminar chromatography technique and gives a brief account of recent applications in which it has been used.

The authors show that the loadability and throughput of ionizable compounds can be enhanced by using hybrid packings.