Data Integrity 2023

Analyzing Organofluorine Compounds in the Environment Using CIC and Other Methods

Part II: Likelihood of Separation, Adjusting Selectivity for Simple Mixtures

 What are Options to Improve My Separation? 

Recognizing Peter Schoenmakers and Emanuela Gionfriddo

The 2023 Lifetime Achievement and Emerging Leader in Chromatography Awards

Analyzing mRNA Therapeutic Drug Substances and Drug Products

The Role of Separation Techniques 

A Look at Ion-Pair Reversed-Phase Chromatography

Improving Chromatographic Results for Oligonucleotides with Column Hardware

This month we interview Petr Vozka from the California State University in Los Angeles, USA, about his work developing methods for analyzing products from plastic waste conversion processes and organic compounds adsorbed on microplastics using two-dimensional gas chromatography (GC×GC).

Vozka, P. Quantitative Analysis of Aliphatic Olefins in Fuels made from Plastic Waste by Comprehensive Two-Dimensional Gas Chromatography. Paper presented at the 14th Multidimensional Chromatography Workshop, Liege, Belgium, 2023.

 is an assistant professor of analytical chemistry in the Department of Chemistry and Biochemistry at the California State University, Los Angeles, USA. He is also the director of the Complex Chemical Composition Analysis Lab (C

AL). His research focuses on characterizing complex chemical mixtures via state-of-the-art techniques, such as two-dimensional gas chromatography (GC×GC) and high‑resolution mass spectrometry (HRMS). His primary focus is on developing methods for analyzing products from plastic waste conversion processes and organic compounds adsorbed on microplastics. He has one patent, six military reports, 25 journal publications, and over 30 research presentations, which include 10 invited lectures and invited presentations. He was invited as a keynote speaker to the 14th Multidimensional Chromatography Workshop in 2023.

Videos

Rising Stars of Separation Science: Petr Vozka

Today’s networking sessions cover a range of techniques and topics. We have two afternoon networking sessions, from 2:00 to 3:30 pm, to recommend. One addresses the impact of automation and AI in the pharmaceutical R&D laboratory (room 202A). The other offers a forum to address the exchange of safety information concerning extractables and leachables (room 203A).

The session on extractables and leachables will be hosted by James Vergis of the Enabling Technologies Consortium and Lee Nagao of Faegre Drinker Biddle and Reath, LLP. This forum will bring together analytical technology providers and industry members to discuss the current extractables and leachables landscape and collaboration opportunities. Topics to be discussed including the current and upcoming regulatory guidance, data sharing, and leachables risk management.

The forum on automation and AI in the pharmaceutical industry will be hosted by Michael Wleklinski of Merck. The group will address how automation and new developments in artificial intelligence can efficiently impact drug candidate progression by using experimental data. Part of this session will also address adopting artificial intelligence technologies into the laboratory, and what challenges and successes attendees have had in doing so.

Articles

On March 21st, Pittcon 2023 has two afternoon sessions that may pique your interest.

Pittcon 2023 – Tuesday Networking Sessions

This afternoon award session, organized by 

 and hosted by Laura Bush, Editorial Director, 

 award winners. The 2023 Lifetime Achievement awardee, Peter Schoenmakers, is a pre-eminent contributor to the development of two-dimensional liquid chromatography (2D-LC), who has also taken on the challenge of three-dimensional liquid chromatography (3D-LC). He is well known for advancing large-molecule separations, particularly polymers, as well as the theory of gradient elution in reversed-phase chromatography and its optimization. The 2023 Emerging Leader, Emanuela Gionfriddo of The University of Toledo, focuses on developing advanced analytical separation tools for the analysis of complex biological and environmental samples using alternative green extraction methodologies. The session will be held in America/Toronto, Room 125.

At 1:30 pm, Laura Bush, Editorial Director of 

 Lifetime Achievement in Chromatography Award to Prof. Peter Schoenmakers of the University of Amsterdam.

At 1:35 pm, Peter Schoenmakers of the University of Amsterdam will give his award presentation on the many challenges he has experienced in researching and developing methods for liquid chromatography. His talk will present some history on how LC has developed, but more importantly will look forward to the challenges in LC that lie ahead. In particular, his talk will address smart approaches to draw meaningful conclusions from the large amounts of data generated from non-target analysis.

Next at 2:10 pm, Bob Pirok of the University of Amsterdam will present his work on the challenges faced for automating 2D-LC. This presentation will discuss the scientific state-of-the-art related to achieving a chemometric method development tool that can be used by public and private organizations for automating the 2D-LC methods.

Beginning at 2:45 pm, Isabelle Kohler, of the Vrije Universiteit Amsterdam (VU Amsterdam), will present detailed research on isomer-specific identification of new psychoactive substances (NPS) using trapped ion mobility mass spectrometry (TIMS-MS). These NPS are structural derivatives of conventional illicit drugs, which are designed to circumvent the law. This presentation describes a novel and fast workflow for high-confidence identification of NPS-related compounds using TIMS-MS.

At 3:20 pm the session will recess until 3:30 pm.

At 3:35 pm, Laura Bush, Laura Bush, Editorial Director of 

 Emerging Leader in Chromatography Award to Emanuela Gionfriddo of the University of Toledo.

At 3:40 pm, Emanuela Gionfriddo of the University of Toledo will present her award presentation on microseparations coupled to chromatographic techniques to unveil potential exposure pathways to environmental contaminants. This presentation will discuss strategies developed in the Gionfriddo’s research group to selectively isolate, preconcentrate and separate micropollutants. This talk will focus on analysis of per- and polyfluorinated alkyl substances (PFAS) and underivatized non-proteinogenic amino acids classified as cyanobacterial neurotoxins.

The final presentation of this awards session is at 4:15 pm when Jim Grinias of Rowan University will present his work to develop a capillary-scale parallel 2D-LC system. This talk will describe detailed efforts toward the development of a capillary-scale comprehensive 2D-LC platform involving unique electronics and instrument feature designs as well as 3D-printed components.

Events

LCGC will be hosting an award session at Pittcon 2023 on March 21st from 1:30 to 4:50 pm, to recognize Peter Schoenmakers and Emanuela Gionfriddo for winning the LCGC Lifetime Achievement and LCGC Emerging Leader Awards for 2023.

LCGC Lifetime Achievement in Chromatography Award and LCGC Emerging Leader in Chromatography Award 

In this morning session, beginning at 8:30 am, Kohei Tsumoto, from The University of Tokyo, and Hiroyuki Kanda, Chief of the Life Science Committee of JAIMA, will preside over the session. The topic of the presented talks involves the need for new methods for the analysis and monitoring of each process from R&D to production of new drug modalities, such as next-generation antibody drugs and nucleic acid drugs. To meet such needs, data science, including AI and machine learning, is being explored to bring new knowledge for understanding of the structure, dynamics, and function of molecules, and for improving the efficiency of drug discovery and research. This session will discuss projects for developing analytical solutions for the pharmaceutical market using new data science improvements. The session will be held in America/Toronto, Room 121C.

At 9:10 am, Noritaka Hashii of the Division of Biological Chemistry and Biologicals at the National Institute of Health Sciences will present the use of the multi-attribute method (MAM) for the evaluation of structural characteristics of therapeutic antibodies. This presentation will describe major modifications of therapeutic monoclonal antibodies (mAbs) characterization and research by the application of MAM analysis.

At 9:45 am, Forrest Kohl, an Application Scientist at JASCO specializing in electronic spectroscopy, will give his talk on an innovative solution for higher-order structure assessment of biopharmaceuticals combining spectroscopy with machine learning and statistical methods. An innovative solution is proposed for the structural analysis of therapeutic antibodies using a new structure estimation algorithm, a spectral similarity evaluation methodology, and Fourier transform infrared (FT-IR), Raman, fluorescence, and ultraviolet-visible (UV-vis) spectroscopy measurements.

At 10:35 am, Zachary Breitbach from AbbVie, Inc. will give a talk on advancing compact liquid chromatography (LC) for elucidating process understanding and for automating analytical instrumentation for biopharmaceutical research and development work. This presentation will report on the development of the Enabling Technologies Consortium (ETC) collaborative advanced compact LC, which is approximately the size of a shoebox, and which was developed by eight pharmaceutical companies partnering with an instrumentation development group. This instrument demonstrates the concept of “sample in – data out” capabilities.

At 11:10 am, Yi-chieh Chen, a Lecturer in the Department of Chemical Engineering at the University of Strathclyde, will present work to develop a spatially resolved spectroscopy (SRS) probe for inline monitoring of the drying process in pharmaceutical drug processing. The talk discusses the developed of an integrated inline Process Analytical Technology (PAT) solution that uses SRS to collect complementary Raman and NIR spectra to determine the sub-surface chemical and physical information for drug samples during production.

On Tuesday morning starting at 8:30 am at Pittcon 2023, a featured session titled, "JAIMA - Analytical Solutions for Research, Development & CMC of Novel Modalities of Drugs: Comprehensive Understanding of Molecular Structure, Dynamics and Function," will take place. 

JAIMA - Analytical Solutions for Research, Development & CMC of Novel Modalities of Drugs: Comprehensive Understanding of Molecular Structure, Dynamics and Function 

Today’s networking sessions cover a range of techniques and topics. For this afternoon, we recommend a forum to address the building of a career as a separation scientist (in room 203A, from 2:00 to 3:30 pm). Amber Hupp of the College of the Holy Cross and Katelynn Perrault of Chaminade University of Honolulu will be hosting the session, which will feature a panel of separation scientists that work in various fields. Panelists will share their insights about what it is like to work in separation science, provide tips and tricks for building your resume, and offer advice for early career scientists. This session is sponsored by the ACS Analytical Division Subdivision of Chromatography and Separations Chemistry (SCSC).

At Pittcon 2023, Amber Hupp and Katelynn Perrault will be hosting a networking session that you won't want to miss.

Building Your Career as a Separation Scientist

This afternoon symposium focuses on opportunities to utilize compact capillary liquid chromatography (LC) techniques in the exploration of drug development. The session will be held in room 118A.

Milton Lee of Brigham Young University will open the session with a talk on the coming of age of compact capillary LC. Lee and his team have developed a hand-portable nanoflow LC instrument that has a small footprint, weighs only 5.4 kg, produces consistent nanoliter to milliliter flowrates at pressures up to 1500 bar, utilizes on-column or miniature flow cell optical UV detection, and is easy to operate.

Next, Samuel Foster of Rowan University will discuss kinetic profiling of capillary LC columns for use in compact LC instrumentation. Foster’s discussion focuses on an integrated column cartridge for use in a compact LC instrument that enables the installation of a wider variety of capillary-scale columns than was previously possible.

Peter Pellegrinelli of Advanced Materials Technology will then address opportunities for developing improved separation methods with modern ultrahigh-pressure LC (UHPLC) instruments. Examples will be shown for coupling a modern UHPLC instrument to new 1.5-mm i.d. columns. By reducing the column i.d. and length, along with making changes in particle size, a chromatographer can reduce solvent usage, decrease run times, and increase sensitivity, an important advantage for sample-limited applications.

Colleen Dugan of GSK will close the session with a presentation on HPLC method-development strategy (MDS) for small-molecule pharmaceutical analysis. In this presentation, the selection process for identifying eight columns to include in an updated MDS toolkit will be described.

At Pittcon 2023, a Monday afternoon symposium will focus on opportunities to utilize compact capillary liquid chromatography (LC) techniques in the exploration of drug development.

Pharmaceutical Analysis Opportunities with Compact Capillary LC

Aaron Acevedo is an assistant editor for LCGC and Spectroscopy at MJH Life Sciences.

This morning session, beginning at 8:30 am, honors Luis Colón of the University at Buffalo, the 2023 winner of the Dal Nogare Award. Presented annually by the Chromatography Forum of the Delaware Valley (CFDV), this award recognizes outstanding scientists who have made great contributions to understanding the chromatographic process. Mary Ellen McNally of FMC will present the award and preside over the session, which will be held in Room 123.

At 8:40 am, Colón will present his study of a diarylethene (DAE) photochromic compound by high-pressure liquid chromatography (HPLC) and/or supercritical fluid chromatography (SFC). For this experiment, Colón and his team studied the formation of a DAE ring-closed isomer via HPLC and SFC. This presentation will show the details of the chromatographic experimental conditions and the findings related to the formation of different DAE isomeric species.

At 9:15 am, Apryll Stalcup of Dublin City University will share tales of her career journey in separation science, in which many blind alleys turned into golden opportunities and new research pathways, in both the United States and Europe.

At 9:50 am, Robert Kennedy of the University of Michigan will present his laboratory’s most recent efforts to push the limits of liquid chromatography (LC) in terms of speed and efficiency. By testing new sample introduction approaches and creating new high-pressure systems, they have been reaching higher efficiencies and improving compound detection in complex mixtures.

At 10:40 am, Susan Olesik of The Ohio State University will share her latest work to improve LC and mass spectrometry (MS) using enhanced-fluidity liquid solvents. Adding carbon dioxide to conventional liquid mobile phases enhances diffusion, decreases viscosity, and maintains mixture polarity, all of which reduces the necessary time needed for analysis. These presented results will illustrate the value of enhanced fluidity liquid mobile phases separations using these retention mechanisms and show the value of these solvents for enhancing mass spectrometric analyses.

At 11:15 am, Stephen Weber of the University of Pittsburgh will discuss the use of electroosmotic perfusion for sampling in the brain. Weber’s laboratory made the discovery that small electrical currents could be used to direct fluid through brain tissue over short distances between a source and a sink, the latter leading to collection or directly to a measurement apparatus. Their most recent work applies two-photon polymerization using a commercial instrument (Nanoscribe) to create devices for passing peptide substrate through tissue in vivo, collecting products, and measuring.

The Chromatography Forum of the Delaware Valley Dal Nogare Award Symposium will take place at Pittcon 2023 on Monday March 20th, starting at 8:30 am. 

The Chromatography Forum of the Delaware Valley Dal Nogare Award Symposium

Laura Bush is a former managing editor of 

. She is currently the Editor-in-Chief of 

. 485 Route One South, Building F, First Floor, Iselin, NJ 08830, lbush@advanstar.com, tel. 732.346.3020

This week at Pittcon, several symposia will honor leaders in separation science. Here is a quick guide.

Sunday afternoon: Satinder Ahuja Award—Emanuela Gionfriddo

This afternoon, Emanuela Gionfriddo of the University of Toledo will receive the Satinder Ahuja Award for Young Investigators in Separation Science, which is given by the ACS Analytical Division Subdivision of Chromatography and Separations Chemistry (SCSC). Talks in this award symposium will be given by:

Emanuela Gionfriddo of the University of Toledo

Susan Olesik of The Ohio State University

Janusz Pawliszyn of the University of Waterloo.

Sunday afternoon: The Pittsburgh Analytical Chemistry Award—Neil Kelleher

Also this afternoon, Neil Kelleher of Northwestern University will receive the Pittsburgh Analytical Chemistry Award. Talks in this award symposium will be given by:

Jonathan Sweedler of the University of Illinois at Urbana-Champaign

Lloyd Smith of the University of Wisconsin–Madison

Luca Fornelli of the University of Oklahoma.

Monday Morning: Dal Nogare Award—Luis A. Colón

Luis A. Colón of the University at Buffalo is the 2023 recipient of the Dal Nogare Award, which is presented by the Chromatography Forum of the Delaware Valley (CFDV). Talks in this award symposium will be given by:

Luis A. Colón of the University at Buffalo

Bob Kennedy of the University of Michigan

Susan Olesik of the Ohio State University

Steve Weber of the University of Pittsburgh.

Tuesday morning: The Pittsburgh Conference Achievement Award—Jeffrey Dick

Jeffrey Dick of Purdue University has won the 2023 The Pittsburgh Conference Achievement Award. Talks in this award symposium will be given by:

Kevin Wilson of Lawrence Berkeley National Laboratory

Christian Chamberlayne of Stanford University.

 Lifetime Achievement and Emerging Leader in Chromatography Awards, presented by 

, are Peter Schoenmakers of the University of Amsterdam and Emanuela Gionfriddo of the University of Toledo, respectively. Talks in this award symposium will be given by:

Peter Schoenmakers of the University of Amsterdam

Isabelle Kohler of the Free University of Amsterdam

 available online or on the “LabSci by Pittcon” mobile app.

Separations Award Symposia this Week

A number of networking sessions are offered this week at Pittcon. Here’s our pick.

On Monday at 2:00 pm, Amber Hupp of the College of the Holy Cross and Katelynn Perrault of Chaminade University of Honolulu will be hosting a networking session titled “Building Your Career as a Separation Scientist” in Room 203A. This networking session will feature a panel of separation scientists that work in various fields. Panelists will share their insights about what it is like to work in separation science, provide tips and tricks for building your resume, and offer advice for early career scientists. This session is sponsored by the ACS Analytical Division Subdivision of Chromatography and Separations Chemistry (SCSC).

On Tuesday at 2:00 pm, James Vergis of the Enabling Technologies Consortium and Lee Nagao of Faegre Drinker Biddle and Reath, LLP, will be hosting a networking session titled, “Extractables and Leachables Safety Information Exchange Analytical Technology Provider and User Forum” in Room 203A. This networking session will bring together analytical technology providers and industry members to discuss the current extractables and leachables landscape and collaboration opportunities. Topics such as the current and upcoming regulatory guidance, data sharing, and leachables risk management will be discussed.

On Tuesday at 2:00 pm, Michael Wleklinski of Merck will be hosting a networking session titled, “The Impact of Automation and AI in the Pharmaceutical R&D Laboratory” in Room 202A. This networking session will address how automation and new developments in artificial intelligence can efficiently impact drug candidate progression by using experimental data. Part of this session will also address adopting artificial intelligence technologies into the laboratory, and what challenges and successes attendees have had in doing so.

On Wednesday at 10:30 am, Perry Wang of the U.S. Food and Drug Administration (FDA) will be hosting a networking session titled, “How to Validate LOQ (limit of quantitation) and Estimate LOD (Limit of Detection)” in Room 203A. This networking session will discuss several terms in the analytical field, including sensitivity, limit of detection, and limit of quantification, with the hope that the discussion will help attendees differentiate these terms better and apply them effectively in their R&D work.

On Wednesday at 10:30 am, Diana Hoover of Seton Hill University will be hosting a networking session titled, “Training the Next Generation of Analytical Chemists: How Many Titrations Do They Really Need?” in Room 202B. This networking session will bring together representatives from undergraduate academic institutions and a variety of industries to answer the most pressing questions related to preparing the next generation of students for the chemical world in which they are destined to work.

A number of networking sessions are offered at Pittcon this year, which will take place March 18–22. Here’s our top picks.

Five Pittcon Networking Sessions We Recommend

Joanna Aizenberg of Harvard University will deliver the 2023 Wallace H. Coulter Lecture at Pittcon. The title of her talk is “Venturing into Analytical Chemistry Using Photonic Crystals.” Her presentation will introduce a powerful colloidal co-assembly approach that creates porous photonic crystals at a large scale. Using selective functionalization of the doped opals, crystal composition and its optical properties can be locally tunable. The liquid-specific wetting behavior exhibited by the crystals naturally couples to macroscopic color changes. Aizenberg will also address in her lecture how the macroscopic color changes can be used to develop simple colorimetric indicators for medical applications and novel, machine learning–enabled artificial nose platforms that utilize biometric approaches. The Wallace H. Coulter lecture will take place at the Michael Nutter Theater.

Aizenberg received her BS in Chemistry from Moscow State University and her PhD in Structural Biology from the Weizmann Institute of Science. After spending close to a decade at Bell Labs, she joined Harvard University as the Amy Smith Berylson Professor of Materials Science and a professor of chemistry and chemical biology.

Aizerberg’s laboratory focuses primarily at understanding biological architecture and how nature solves complex problems in the design of multifunctional, adaptive materials. Using basic principles of biological architecture as a guide, this knowledge is then applied to develop new synthetic routes and nanofabrication strategies that can lead to technological advances in devices used in various applications, such as biometrics, energy, and medicine.

Aizerberg has been elected to various societies, including the National Academy of Sciences, the National Academy of Engineering, the American Academy of Arts and Sciences, the American Philosophical Society, and the American Association for the Advancement of Science. She is a Fellow of the American Physical Society and the Materials Research Society and an external member of the Max Planck Society. Aizenberg’s select awards include the MRS Medal, the Kavli Innovations in Chemistry Leader Award, the Fred Kavli Distinguished Lectureship in Nanoscience,the Ronald Breslow Award for the Achievement in Biomimetic Chemistry, and Harvard’s Ledlie Prize for the most valuable contribution to science. She has more than 280 publications and 90 issued patents, and she is a founder of four start-up companies.

The lecture will be followed by the Pittcon Welcome Reception. 

Joanna Aizenberg of Harvard University will deliver the 2023 Wallace H. Coulter Lecture at Pittcon. The title of her talk is “Venturing into Analytical Chemistry Using Photonic Crystals.” 