Application Notes: Sample Prep

Evaluation of Mixed-Mode Ion Exchange SPE for the Extraction of Opioids from Human Urine by UHPLC-MS/MS

April 25, 2022

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This application notes introduces an SPE LC–MS/MS methodology using the Microlute™ CP SCX SPE 96-well microplate for the determination of 12 opioids from human urine.

Application Note: Hydrocarbon Fractionation Using Enviro-Clean Fusion®Ag+

April 22, 2022

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This application note outlines the fractionation of hydrocarbons into aliphatic and aromatic fractions using UCT’s new silver-phase EPH fractionation cartridges.

Extractions of THC-metabolites from Urine

April 19, 2022

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This application note demonstrates a sample preparation procedure for using the Empore C18 SPE cartridge for the extraction of the THC metabolite, THC-COOH, from urine samples.

Selection of UHPLC Columns for Nucleic Acid Oligomer Analysis

April 18, 2022

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Synthesized 40-mer miRNA has been analyzed using both a conventional column and new types of UHPLC columns.

Scaling for Efficiency and Throughput: LC Method Optimization with Notes on Selectivity and Preparative LC

April 15, 2022

Executive Summary

MHC-Associated Peptide Purification for Immunopeptidomics

April 13, 2022

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Automated MHC-I is associated peptide enrichment using the Agilent AssayMAP Bravo platform with large-capacity Protein A cartridges for immunopeptidomics analysis.

Quantification and Characterization of Biotherapeutics Proteins

April 13, 2022

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The AssayMAP Bravo was used to purify, digest, and clean up antibodies from 96 cell culture supernatants with high reproducibility and minimal labor prior to LC–MS.

Intact Antibody and Subunit Mass Determination

April 13, 2022

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Intact and subunit antibody mass determination was streamlined using automated purification and enzymatic cleavage and deglycosylation of immobilized antibodies.

Assessing Multiple CQAs for Monoclonal Antibodies v2

April 13, 2022

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Automated sample prep and LC–MS peptide mapping approach to characterize and quantify multiple monoclonal antibody (mAb) critical quality attributes (CQAs)

Automated Phosphopeptide Enrichment from Complex Mixtures

April 13, 2022

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Automated phosphopeptide enrichment from cell lysates with Fe(III)-NTA enables the identification of thousands phosphopepides with high reproducibly and selectivity.