Application Notes: Sample Prep

How to Avoid Contamination in Pipetting EU

April 02, 2021

Avoiding Depurination During Trityl-on Purification

March 18, 2021

This technical note explores a new trityl-on procedure that minimizes the risk of depurination.

Clarity OTX Application Guide

March 18, 2021

Extraction of oligo therapeutics from biological samples for LC–MS bioanalysis

Extraction of 2’-MOE Phosphorothioate from Plasma Using Clarity OTX

March 18, 2021

Using the Clarity OTX extraction protocol vendor recommendations, we have demonstrated its use to develop a method for the extraction of therapeutic oligos from plasma.

RNA Sample Preparation and High Throughput Purification for TBDMS and TOM Chemistries

March 18, 2021

This technical note discusses 2' cleavage and RNA sample preparation prior to trityl-on Clarity QSP cartridge or high-throughput purification.

Automated Method Development of Oligonucleotide in Tissues Using Clarity OTX 96-Well Plate and High-Resolution Mass Spectrometry

March 18, 2021

As synthetic oligonucleotide therapeutics traverse the drug-development path, there is an increasing need for TK and PK/PD studies in drug discovery and development.

FAQs for the Isolation and Purification of Synthetic Oligonucleotides

March 18, 2021

Common frequently asked question for oligonucleotides. This includes questions about characterization to purification and from sample preparation to LC separation.

Extraction and LC/MS Analysis of Oligonucleotide Therapeutics from Biological Matrices Using Clarity® OTX™ SPE and Clarity Oligo-MS™ HPLC/UHPLC Columns

March 18, 2021

This technical note discusses a simple, yet quick and effective solution for isolating and analyzing therapeutic oligonucleotides using Clarity OTX, an extraction kit.

Clarity Biosolutions for Synthetic DNA/RNA Guide

March 18, 2021

Advance your oligonucleotide work, from sample preparation to analysis and purification

Extraction of 2’-MOE Phosphorothioate from Tissue Using Clarity OTX Solid Phase Extraction (SPE)

March 18, 2021

To ensure recovery and reproducibility for oligo extraction from tissue, it is imperative that an effective sample pretreatment strategy is used.