Application Notes: LC-MS

PFAS rules now reach the packaging itself. Under the EU Packaging and Packaging Waste Regulation, food contact packaging containing 25 ng/g or more of any individual PFAS cannot be placed on the market, so labs need targeted data at production volume. This application note shows a Direct Shoot LC-MS/MS method on the PerkinElmer QSight 500 system that quantifies 26 PFAS in food packaging bottles, pharmaceutical packaging bottles and packaged drinking water. It uses a simple methanol extraction and no SPE cleanup. Calibration runs from 0.05 to 25 ng/g, with recoveries predominantly within 70 to 130%.

Learn how water quality, handling, purification technologies, final filters, and maintenance practices help ensure optimal LC-MS performance and reliability

Corteva accelerates chromatography method development using AutoChrom to automate workflows, improve efficiency, and reduce method development time.

How Pfizer improved HPLC method transfer outcomes using method development software to enhance consistency, efficiency, and reproducibility in workflows across labs.

Merck streamlines chromatographic method development using in silico modeling to improve efficiency, reduce experimentation, and accelerate decision-making.

Ensure confident PFOS detection in complex food matrices with advanced LC-MS separation using the bioinert YMC Accura Triart C18 column.

Learn how proper water purification eliminates PFAS background, enabling ultra sensitive LC MS/MS analysis with confidence using EPA 1633 ready ultrapure water outputs.

A practical guide to LC–MS calibration, comparing external, surrogate and in sample approaches to control matrix effects and support accurate, regulator aligned bioanalysis.

This application note highlights a new multi-modal LC-MS/MS approach enabling robust analysis of polar and ionic metabolites without derivatization or ion-pairing.

Separations of Oligonucleotides using HALO 1000 Å OLIGO C18 show improved peak capacity, narrower peaks, and lower backpressure vs. 300 Å FPP columns.

HALO 1000 Å OLIGO C18 enables high-resolution impurity analysis of 90-mer ssDNA, outperforming competitors in peak capacity and separation efficiency.

Larger pore columns improve efficiency and reduce band dispersion in oligonucleotide separations-especially at high flow rates and longer chain lengths.

Longer columns paired with scaled gradients enhance oligonucleotide resolution especially for complex mixtures up to 100 nucleotides in length.

Discover how HALO® OLIGO C18 columns improve DNA/RNA separation with optimized ion pairing, pH control, and particle morphology for faster, sharper results.

Improve analysis of betamethasone derivatives with CORTECS™ Premier C8 Columns featuring MaxPeak™ HPS Technology for sharper peaks and better analytical performance.

Improve validated HPLC methods by switching from fully porous to CORTECS™ Premier C18 5 µm Columns to boost throughput, cut solvent use, and enhance method greenness.

This application note describes the separation of the 20 standard PFAS according to Directive (EU) 2020/2184 in only eight minutes.

n this application note, a USP monograph is modernized to a shorter column using 5 µm particles, which can be allowable under USP <621> as long as the N value is within the guidelines. By using highly efficient CORTECS 5 μm particles, this type of modernization is possible and reductions in solvent usage and run times are achieved

In this work the development of two key attributes of the 5um CORTECS Columns is examined. First column efficiency is compared across CORTECS and other solid-core 5 μm columns. Next scalability from sub-2 μm to 5 μm particles is examined between the CORTECS Column lines and competitive column lines. It was found that CORTECS columns have higher efficiency compared to other solid-core columns and that CORTECS particles are fully scalable.

Ultrashort-chain PFAS (C1–C4) are important due to their widespread presence and here is an application solution for this analysis.

Slalom chromatography separates nucleic acids >3 kbp in <6 minutes using shear-based molecular stretching for high-resolution, gel-free analysis.

he United States Pharmacopeia (USP) has designations for all columns stationary phases used in the monograph methods. These designations outline the stationary phase type, i.e. fully porous or solid-core, and any ligand attachments, i.e. C18 or Phenyl to be used.1 However, beyond that no column specifics are given. With a multitude of columns that fit into the different designations, understanding that not all columns are the same is vital when selecting a stationary phase for a monograph method. This application note examines three columns that all fit into the L1 designation when analyzing paracetamol impurities. Selectivity differences between the columns are considered in relation to the impurities.

Explore how wide-pore SEC delivers sharp, reproducible separation of megadalton-sized DNA vectors and plasmids with minimal secondary interactions.

We evaluated the accuracy of lipid quantitation in human plasma by LC-MS using single-point or multipoint calibration curves with authentic or surrogate standards.

This SEC-MS method demonstrates the precise separation of a single-chain variable fragment from its drug-conjugate.

In this application note the charge variant characterisation of IgG4-based mAbs was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

This application note provides you an optimum RP method for the DAR determination of a site-specific ADC using a widepore YMC-Triart Bio C4 column.

This application with data from Roche Diagnostics confirms superior performance of bioinert-coated YMC Accura BioPro IEX SF columns for the IEX-MS analysis of mAbs.