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© 2023 MJH Life Sciences™ and Chromatography Online. All rights reserved.
December 15, 2022
Although the majority of benzodiazepines metabolize to a single compound, oxazepam, other metabolites can be detected by liquid chromatography.
In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.
The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.
Paclitaxel originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.
The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple, efficient identification of the whole vitamin series (K2, K3, and K4).
A Hamilton PRP-C18 (5 μm) HPLC column was used for purification of three oligonucleotides. Analysis of a 21-mer, 22-mer, and 23-mer of similar structure was evaluated.
December 08, 2022
The primary nucleosides encompass a broad array of biological functions associated with RNA, particularly after configuration into multidimensional conformations.
December 06, 2022
This application note describes a 2D-LC workflow for Protein A purification of mAbs, followed by online reduction and MS analysis of mAb subunits.
November 30, 2022
A separation of parabens is performed on a HALO 90 Å LPH-C18 column under low pH (pH 1) and high temperature conditions compared to a standard C18 SPP column.
A low pH compatible, 90 Å, superficially porous particle C18 phase is introduced as an addition to the chromatographer’s toolbox.