Application Notes: LC

Benzodiazepine Metabolites by Reversed-Phase HPLC

December 15, 2022

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Although the majority of benzodiazepines metabolize to a single compound, oxazepam, other metabolites can be detected by liquid chromatography.

Separation of Basic Drug Compounds

December 15, 2022

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In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.

Raffinose Family of Oligosaccharides by Cation Exchange

December 15, 2022

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The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.

Pick Your Poison. Isolation of Paclitaxel

December 15, 2022

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Paclitaxel originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

Separation of Fat Soluble Vitamin K Congeners in Under 6 Min

December 15, 2022

The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple, efficient identification of the whole vitamin series (K2, K3, and K4).

Oligonucleotide Purification by Reversed-Phase Ion Pairing

December 15, 2022

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A Hamilton PRP-C18 (5 μm) HPLC column was used for purification of three oligonucleotides. Analysis of a 21-mer, 22-mer, and 23-mer of similar structure was evaluated.

Separation of 12 Nucleosides on Hamilton PRP-1 Column (Dec 2022)

December 08, 2022

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The primary nucleosides encompass a broad array of biological functions associated with RNA, particularly after configuration into multidimensional conformations.

Automated Monoclonal Antibody Subunit Analysis

December 06, 2022

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This application note describes a 2D-LC workflow for Protein A purification of mAbs, followed by online reduction and MS analysis of mAb subunits.

Low pH Stability with HALO LPH-C18

November 30, 2022

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A separation of parabens is performed on a HALO 90 Å LPH-C18 column under low pH (pH 1) and high temperature conditions compared to a standard C18 SPP column.

HALO LPH-C18: Enhanced Stability for Low pH Applications

November 30, 2022

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A low pH compatible, 90 Å, superficially porous particle C18 phase is introduced as an addition to the chromatographer’s toolbox.