Application Notes: LC

HPLC is the method of choice for enhancing the purity of synthesized peptides. Here, a systematic and efficient workflow for scaling up peptide purification is shown.

Polysome profiling is a method for analysing the translational status of cells. This AppNote describes a simple FPLC system for polysome fractionation.

Organic solvent, pH, salt concentration, and gradient mixing rates influence HIC separations. Optimizing these factors allows for effective separation of mAbs and ADCs.

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

The identification benzodiazepine metabolites can be highly valuable in detecting abuse. Metabolites of oxazepam are found containing both hydroxyl and glucuronides.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions.

The fast identification of tocopherols are important for various applications including free radical scavenging for human health and food stuff preservation.

Isolation of capsaicin for medicinal uses is easily achieved with the PRP-C18 RP-HPLC resin.

Discover how the HC-75 series uses a ligand exchange mechanism in order to interact more or less strongly with the analytes of interest.

This brief communitation hightlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

This application note introduces our simple and effective KNAUER HPLC Method Converter for modernising analytical methods according to the allowed USP 621 variations.

Application Notebook highlighting Liquid Chromatography techniques commonly used for monitoring Critical Quality Attributes (CQAs) in Bioprocessing Applications

Strategies to screen and develop a preparative HPLC method and its up-scaling for purification of CBD from Cannabis extract

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

Garlic has been found to exhibit a multitude of proliferative health benefits in the realm of cardiac events.

A reversed-phase HPLC method was developed to separate five steroid hormones, with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).

Sulfide compounds found in garlic oils are used in therapeutics and prophylaxis of certain cancers, and are prevalent in cell health.

Identification of the five most common saccharides found in packaged foods, lactose, maltose, sucrose, fructose, and glucose, is easily resolved on the RCX-30 column.

The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.

Isolation of six common allergens can be achieved by utilizing reversed-phase HPLC using an alkaline eluent.

With the growing utility of the K vitamin series of molecules, a need appears relevant for both food labeling of constituents and for research based separation of the natural and synthetic compounds of vitamin K. The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple efficient identification of the whole vitamin series (K2, K3, and K4).

Experience high recoveries from the first injection! YMC Accura BioPro IEX SF columns provide excellent peak shapes for the ion-exchange analysis of mAbs.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

This application note demonstrates the potential of valorizing mother liquor using Centrifugal Partition Chromatography (CPC) to remediate minor cannabinoids.

This application note discusses the latest development on high performance cation exchange bio-separation columns for determining charge variants in protein-based biotherapeutics, as well as the strategy on method development.

In this application note the chelating agents 8-hydroxyquinoline and hinokitiol were analyzed using a bioinert coated YMC-Accura Triart C18 column.

Experience high recoveries from the first injection! YMC Accura BioPro IEX SF columns provide excellent peak shapes for the ion-exchange analysis of mAbs.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

A new technical study demonstrates the utility of a non-invasive liquid flowmeter for validating the performance of HPLC systems across a range of 9 different solvents.

We assess the benefits of the Alliance iS Bio HPLC System by migrating and modernizing a compendial SEC method outlined in the USP General Chapter <129>.