HPLC

High performance liquid chromatography (HPLC) is a widely used and well-established technique, routinely employed by thousands of analytical scientists worldwide. Nonetheless, certain challenges—arising from the complex interplay of multiple factors affecting peak retention and separation—persist. In this context, multidimensional modeling approaches can provide valuable support.

A recent study demonstrated how analytical techniques can be used to differentiate between different types of whiskey.

Researchers at the University of Huddersfield analyzed 60 new and used disposable e-cigarettes using HS-GC–MS and HPLC–DAD, finding concerning levels of ethyl maltol and benzoic acid and detailing their chemical composition and changes with use.

In this installment, we explore the key relationships between efficiency and mobile phase flow rate and particle size, as well as secondary determinants of efficiency, including analyte retention, size, and chemistry, and instrumental effects.

Curcumin is a polyphenol believed to have anticancer effects. To better quantify its existence in plasma, reversed-phase high-performance liquid chromatography (RP-HPLC) was used to create a new analytical approach for quantifying this substance.

To better detect cardiovascular diseases in people, researchers used high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) to simultaneously quantify metabolites in human plasma.

Johns Hopkins University researchers, in response to many high performance liquid chromatography (HPLC) systems having issues with peak resolution, created their own system for quantifying complex HPLC chromatograms.

In this article, you’ll find some of the top content that was published on LCGC this week, including details on the recent merger between Waters Corporation and BD Biosciences.

Recently, we spoke with Esther Olonimoyo from the University of Maryland, who recently published a study using high-performance liquid chromatography to analyze short chain fatty acids.

In a new study, researchers explored a new approach for detecting pesticide residues in water samples, based around ionic liquid-based dispersive liquid–liquid microextraction (IL-DLLME).

This review highlights the high performance liquid chromatography (HPLC), mass spectrometry (MS), chromatography data systems (CDS), and related products that were introduced from 2024 to 2025, summarizing the technical specifications of each product and highlighting significant user benefits.

Microplastics can linger in aquatic ecosystems, and there are limited studies that evaluate those substances’ sorption kinetics. Researchers aimed to investigate the sorption behavior of cortisone on MPs.

Oxygen heterocyclic compounds are notable components in citrus-scented creams, though quality control is important when using these compounds. Researchers recently created a workflow for extracting these substances using a deep eutectic solvent (DES) for microwave-assisted extraction (MAE).

In this month's edition of the LCGC Blog, discover how weighted least squares regression enhances quantitation accuracy in analytical chemistry, particularly for low-concentration analytes in HPLC.

Isavuconazole, an antimycotic agent used to treat fungal infections, can typically be found during dried blood spot sampling. However, there are obstacles that keep it from being an ideal approach for properly determining the drug’s presence.

Brett Paull from the University of Tasmania, Tasmania and his team describe the latest development in portable LC instruments and their experience of taking portable systems out to the field.

High-performance liquid chromatography (HPLC) was recently used to detect acylated impurities in artificial hormones meant to treat prostate cancer symptoms.

The 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025) will be held from Sunday through Thursday, 15–19 June, 2025, in Bruges, Belgium.

Ceftazidime and avibactam are two anti-infective drugs that can prove difficult to analyze in serum. Determining them can assist in therapeutic drug monitoring or pharmacokinetic studies.

Flavonoids are widely used metabolites that carry out various functions in different industries, such as food and cosmetics. Detecting, separating, and quantifying them in fruit species can be a complicated process.

Highly reactive aldehydes can pose significant health concerns in polluted environments. HPLC was used in this study to simultaneously analyze carbonyl compounds hydrazones.

University of Caldas and University of Chile researchers extracted estrogenic hormones from wastewater samples using rotating disk sorption extraction. After extraction, the concentrated analytes were measured using liquid chromatography coupled with photodiode array detection (HPLC-PDA).

Led by Esther A. Olonimoyo, University of Maryland researchers created a new HPLC-based method for analyzing short-chain fatty acids.

Here is some of the most popular content posted on LCGC International this week.

Scientists from Ferdowsi University tested a new type of sorbent for effectively extracting remdesivir from samples.