Author
Latest:
In Memory of Pavel Jandera, Leading ChromatographerProfessor Pavel Jandera passed away suddenly and unexpectedly on August 5, 2021. He was one of the most famous Czech analytical chemists. He was 77 years old.
Latest:
Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography MethodsA column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
Latest:
An Overview of the Untargeted Analysis Using LC–MS (QTOF): Experimental Process and Design ConsiderationsUntargeted analysis using liquid chromatography with tandem mass spectrometry (LC–MS/MS) chemical profiling is a valuable tool, but care needs to be taken with experimental design.
Latest:
Rapid Simultaneous Determination of Five Major Alkaloids from Menispermi Rhizoma in Rat Urine by Ultrahigh-Pressure Liquid Chromatography– Tandem Mass Spectrometry (UHPLC–MS/MS) and its Application to a Urinary Excretion StudyA UHPLC–MS/MS method is described for rapid quantification of five major bioactive alkaloids in rat urine. The results obtained help lay the foundation for the clinical application and safety evaluation of the bioactive ingredients of menispermi rhizoma, used in herbal medicines.
Latest:
PFAS Testing Solution GuideCheck out the testing solution guide for analyzing PFAS in water, soil and air. This guide provides chromatography consumables for reliable PFAS testing.
Latest:
Py-GC-MS Investigation of Pyrolysis Behaviours and Decomposition Products of α- and β-2,7,11-Cembratriene-4,6-DiolsThe study of the thermal behaviour and pyrolysis products of these terpenoids could possibly suggest flavour precursors that could be used to provide specific flavours to tobacco.
Latest:
Liquid Chromatography–Mass Spectrometry Analysis of Hop-Derived Humulone and Isohumulone Constituents in Beer: The Bitter Truth of Hops Utilization During BrewingA rapid LC–MS method using ESI coupled with SIM-MS for analysis of humulone and isohumulone content in beer is described.
Latest:
Essentials of LC Troubleshooting, Part II, Continued: More Misbehaving Retention TimesWhen can analyte retention deviate from what is expected or normal? We explain three subtle causes.
Latest:
Wastewater Surveillance of Pharmaceuticals and Genes: Challenges and OpportunitiesSample preparation and analysis of pharmaceuticals in wastewater present unique challenges. Here, we describe those challenges.
Latest:
Comparison of PFAS Sample Preparation Between WAX + Dispersive GCB and WAX/GCB Cartridges from Water and Soil Extracts Per Draft EPA 1633How to speed up sample prep time and equivalent acccuracy and precision.
Latest:
Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography MethodsA column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
Latest:
A Multiphase Sampling and Analytical Approach for Investigating Airborne PFAS TransmissionThe investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.
Latest:
Analysis of UV-Treated Mushrooms: Dietary Source of Vitamin D2?In our study, a fast and sensitive liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) method for the determination of vitamin D2 in fresh mushrooms and its metabolite 25(OH)D2 in the blood of volunteers regularly consuming UV-treated mushrooms has been introduced.
Latest:
A Suitable Therapeutic Drug Monitoring Method for Amoxicillin in Plasma by High Performance Liquid Chromatography–UV (HPLC–UV) in NeonatesThis simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.
Latest:
Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry PerspectiveThis review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
Latest:
Analytical Techniques in Exploring the Persistent Global PFAS ProblemThe presence of PFAS in the environment requires governmental agencies to establish regulatory limits for PFAS in human essentials, such as drinking water. LC–MS/MS can be used to analyze a wide range of sample types containing PFAS, but avoiding PFAS contamination is critical for this technique to work effectively.
Latest:
Sustainability in Pharmaceutical Separation Science: A Virtual SymposiumWebinar Date/Time: Wednesday, April 19, 2023
Latest:
Groundwater CSI: Unravelling Pollution Sources in Complex Environments with Liquid Chromatography Triple Quadrupole Mass SpectrometryIt is often very difficult to distinguish the true source of wastewater contamination, especially where wastewater treatment plants are located in agricultural areas. Robust and sensitive techniques are needed to characterize impacts where there are multiple potential sources. This study analyzed a variety of synthetic chemicals using LC–QQQ–MS to generate unique fingerprints of pollution.
Latest:
Stability and Recovery Influences of Benzo[a]pyrene, Benzo[a]anthracene, Benzo[b]fluoranthene, and Chrysene during Sample Preparation of Plant MatricesQuantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane–ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false results. In the case of benzo[a]pyrene with a deviation of 155% from the theoretical true value.
Latest:
Exploring the Implementation of Compact Chromatographic Instrumentation in Common Analytical WorkflowsCompact instrumentation offers important advantages for many workflows, as illustrated by these examples.
Latest:
Biomimetic HPLC Methods for Drug DiscoveryModified nucleosides, either natural or synthetic, often provide RNA with distinctive properties useful in diagnostics or therapeutics.
Latest:
Analytical Advantages of Highly Stable Stationary Phases for Reversed-Phase LCDuring the past five years, many manufacturers of HPLC columns have focused on improving stationary phase stability and reproducibility. In this study, the authors use a variety of test solutes to compare the efficiency, selectivity, and hydrophobic retention mechanisms of five commercially available HPLC columns based silica, alumina, zirconia, and polystyrene cross-linked with divinylbenzene as the support.
Latest:
The 15th Multidimensional Chromatography WorkshopThe 15th Multidimensional Chromatography Workshop is a free event involving keynote and contributed presentations, a poster session, and discussion groups on all multidimensional techniques, and will be held in-person on the campus of California State University, Los Angeles, from January 10 to 12, 2024.
Latest:
Separation Science: The State of the Art: Field-Flow Fractionation: Extended Frontiers to Supramolecular and Complex Polymer MaterialsIn this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
Latest:
Improving Selectivity in the Chromatographic Analysis of Monoclonal Antibodies (mAbs) Through the Use of Multi-Isocratic Elution ModeMulti-isocratic elution mode is a powerful chromatographic approach for characterizing minor isoforms of monoclonal antibodies.
Latest:
Application of Porous Layer Open Tubular Columns: Beyond Permanent GasesThe application of porous layer open tubular (PLOT) columns can be expanded beyond light compounds to separations of analytes with heavier boiling points.
Latest:
Determination of Microplastic Mass Content by Thermal Extraction Desorption Gas Chromatography–Mass SpectrometryA novel analysis technique has been developed for the determination of microplastics (MPs) in complex environmental samples using thermal extraction desorption gas chromatography–mass spectrometry (TED-GC–MS).
Latest:
Method Development for the Qualitative and Quantitative Analysis of Pesticides by Direct Injection Liquid Chromatography– Tandem Mass Spectrometry (LC–MS/MS)This modification of ASTM method D8026 for pesticides in environmental matrices includes more pesticides and lowers the reporting limits, thus increasing throughput and measurement capacity for a large surface-water monitoring project.
Latest:
A Flash Qualitative Identification Method for the Specific Component in a Mixture Based on Diode Array DetectorThis paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.
Latest:
Possibilities and Limitations of Aqueous Temperature Responsive Liquid Chromatography in Comprehensive 2D–LCAnalyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.
