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The Ever-Changing World of Extractables and Leachables: Lessons learned and future challengesOctober 4, 2021 at 3pm BST | 10am EST | 4pm CEST Join global industry and regulatory experts as they discuss current hot topics in extractables and leachables (E&L) testing for pharmaceuticals, biopharmaceuticals and medical devices.
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Six Key Differentiators Between Liquid Chromatography and High-Resolution Ion MobilityUsing ion mobility, analytes that have the same molecular mass can be separated by their shape, centers of mass, and collision cross section, but challenges such as ion loss can still occur. A new development in ion mobility separation, high-resolution ion mobility (HRIM), addresses such problems, and is particularly well suited to challenging applications, such as glycosylation monitoring of biological drugs and vitamin D analysis.
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Dispersive Solid-Phase Extraction and Solid-Phase Extraction for ppt-level PFAS Analysis in Apples: A ComparisonPer- and polyfluoralkyl substances (PFAS) are found in our food. Sensitive, precise, and accurate analytical methods are needed to estimate human exposure to these chemicals. A comparative study was performed between two extraction and cleanup methods (solid-phase extraction [SPE] and dispersive SPE) for the analysis of PFAS in apples. Both methods showed excellent sensitivity, precision, and accuracy. dSPE has some benefits over conventional SPE, and vice versa. The advantages and disadvantages of both methods are discussed.
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Troubleshooting LC Separations of Biomolecules, Part II: Passivation and Mobile-Phase AdditivesIf you are analyzing metal-sensitive biomolecules, and a bioinert instrument is unavailable, or insufficient, passivation or mobile-phase additives may help. Here’s how to use those solutions, with tips for avoiding potential pitfalls.
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Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography MethodsA column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
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Effectiveness of High-Resolution Ion Mobility for Complex AnalysesHRIM has emerged as a robust separation strategy for complex chemical analyses due to its ability to improve peak capacity and aid in the separation of isobaric signals.
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Identification of Microplastics in Environmental Monitoring Using Pyrolysis–GC–MS AnalysisPyrolysis–gas chromatography–mass spectrometry has advantages for the analysis of environmental microplastic samples compared to other leading analytical methods, including spectroscopic techniques.
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Optimization of Determination Method of Cooling Agents in Cigarette Tipping Paper by Gas ChromatographyThis GC–FID method enables accurate determination of cooling agents, which are flavor compounds commonly used in cigarette tipping paper.
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Comprehensive Gas Chromatography Coupled to Simultaneous Dual Detection (TOF-MS/FID) as a Confirmatory Method for MOSH and MOAH Determination in FoodFood contamination from mineral oil saturated hydrocarbons (MOSHs) and mineral oil aromatic hydrocarbons (MOAHs) is problematic and requires a sensitive analytical technique. These contaminants were analyzed using GC×GC with flame ionization detection (FID) and time-of-flight–MS (TOF–MS) parallel dual detection. The method provides enhanced chromatographic separation, along with the full mass spectra information, and overcomes difficult interferences, resulting in reduction of false positives over conventional GC–MS methods.
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Evaluating Two Dioscin-Based Silica Stationary Phases and their Application to Achiral and Chiral SeparationsImproved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.
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Purification and Isolation of Cannabinoids: Current Challenges and PerspectivesThe current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.
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Anomalous Retention Prediction Using Modelling Software in Gradient Reversed-Phase Liquid Chromatography: Why it Can Occur and How to Prevent ItThe success of screening column and mobile phase combinations that generate dissimilar selectivity is highlighted in a typical method development strategy.
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Supplementary Info: Non-aqueous Ion-Exchange Chromatography Using High Acid‑base Concentration: A Strategy for Purifying Non-crystalline Pharmaceutical IntermediatesThis information is supplementary to the article “Non-aqueous Ion-Exchange Chromatography Using High Acid‑base Concentration: A Strategy for Purifying Non-crystalline Pharmaceutical Intermediates” that was published in the March 2022 issue of LCGC Asia Pacific.
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Multi-Active Method (MAM) for the Analysis of Agriculture Product Technical Ingredients and Formulated ProductsA collaborative multi-analyte method developed utilizes HPLC and UHPLC to analyze more than 70 active ingredients. This method offers a validated approach for determining technical AI alongside linearity, precision, accuracy, and specificity tests for seven active ingredients.
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Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry (HS-SPME-GC–MS) for the Characterization of Cigar LeavesHS-SPME-GC–MS was combined with OPLS-DA data analysis to tentatively identify eight chemical markers to differentiate the geographical origins of cigar leaf samples.
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Overcoming Incomplete Peptide Mapping of Antibody Complementarity-Determining Regions with Alternate Digestion WorkflowsPepsin digestion and guanidine hydrochloride post-digestion can improve sequence coverage in antibody peptide mapping compared with trypsin digestion.
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Functional Copolymers Analysis Using Liquid Chromatography (LC)—Sort It OutThe latest developments and challenges of using liquid chromatography (LC) for copolymer analysis are discussed.
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Simple Purification and Ultrahigh-Pressure Liquid Chromatography–Tandem Mass Spectrometry Determination of Five Quinoxaline 1,4-dioxides and their Metabolites in Swine LiverThe UHPLC–MS/MS method can accurately determine the presence of these illegal feed additives in swine tissues.
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Purification and Isolation of Cannabinoids: Current Challenges and PerspectivesThe current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.
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Cannabis Metabolomic Data Processing: Challenges to be AddressedThe challenges that arise during cannabis metabolomics analysis using ultrahigh-performance reversed-phase liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC–reversed-phase–HRMS/MS) are presented.
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Exploring the Implementation of Compact Chromatographic Instrumentation in Common Analytical WorkflowsCompact instrumentation offers important advantages for many workflows, as illustrated by these examples.
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Achieving Optimum GC Separation – Designing New Capillary Column Stationary Phases Using Computer ModelingBy calculating optimal stationary-phase composition and physical parameters, column developers can construct columns that provide the exact separation requirements desired by analysis or current methods.
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Indirect Suppressed-Conductivity Ion ChromatographyThe authors demonstrate a new detection method for various anions of weak acids separated by ion chromatography and point out the main advantages of this method.
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Optimizing PFAS Sample Preparation Efficiency and Laboratory Cleanliness for EPA Method 1633U.S. Environmental Protection Agency (EPA) Method 1633 signifies a pivotal shift in per- and polyfluoroalkyl substance (PFAS) determination, extending beyond drinking water.
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Quantifying Smoke Taint in California Wines by Immersive Sorbent Sheet Extraction Prior to Direct Analysis in Real-Time Mass Spectrometry (DART-MS)Measuring volatile phenols in wine is essential in ensuring superior wine quality. A new analytical technique, called solid-phase mesh-enhanced sorption from headspace (SPMESH), was modified with direct immersion (DI) conditions and coupled to direct analysis in real time–mass spectrometry (DART–MS) to be used to detect smoke taint in winemaking.
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Achieving Optimum GC Separation – Designing New Capillary Column Stationary Phases Using Computer ModelingBy calculating optimal stationary-phase composition and physical parameters, column developers can construct columns that provide the exact separation requirements desired by analysis or current methods.
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Validation of HPLC-DAD Based Stability Indicating Assay Method for Ornidazole in Periodontal Polymeric Hydrogel: Robustness Study using Quality by Design (QbD) ApproachIn the present study, a gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method has been designed and validated to quantify ornidazole (OZ) in the marketed formulation (oral gel) with the application of QbD.
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Approaches to Accelerate Liquid Chromatography Method Development in the Laboratory Using Chemometrics and Machine LearningThe authors explore computer-aided workflows and machine learning, aimed at optimizing LC parameters, focusing on kinetic and thermodynamic aspects, and proposes closed-loop optimization strategies.
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Real-Time Stack Emission Measurements of Non-Traditional Odorous Species from an Industrial Processing FacilitySelected ion flow tube mas spectrometry (SIFT–MS) offers real-time measurements directly from air without chromatography or sample preparation, providing an attractive alternative for these hard-to-measure volatile organic compounds.
