Biopharmaceutical analysis is being asked to keep pace with increasingly complex molecules, from monoclonal antibodies to oligonucleotides, lipid nanoparticles, and RNA therapeutics, while method development itself becomes faster and more automated. In Advances in Biopharmaceutical Analysis, LCGC International brings together leading researchers tackling that challenge: revisiting the history, principles, and growing applications of affinity chromatography, exploring how AI and robotics are reshaping method development in the autonomous laboratory, automating the Separation Quality Factor (SQF) within chromatography data systems for objective method evaluation, and combining solid-phase enzymatic digestion with porous graphitic carbon chromatography for rapid LC–MS analysis of RNA modifications.