Fully Automated Two-Dimensional LC Protein Separation using Monolithic Columns - Dionex Apps Note

Separation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.

In this month’s column, I highlight some of the primary considerations we face in method development and point to resources that can help users overcome uncertainty and develop highly effective 2D-LC methods.

During the 18th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology, experts presented a workshop on different ways multidimensional liquid chromatography can be applied.

Here are the top five articles that the editors of LCGC International published this week.

At Analytica 2024 in Munich, Germany, LCGC International sat down with Sebastiaan Eeltink of Vrije Universiteit Brussel to talk about three-dimensional liquid chromatography (3D–LC) separations.

At HPLC 2023, John Langley, a professor at the University of Southampton in Southampton, UK, presented a talk called "(R)evolution of Hyphenations, Through Open-Access RP-HPLC–MS, Open-Access UHPSFC–MS and 2D-LC–IMS-MS in Academia". He spoke with LCGC on the importance of open-access hyphenation, and how to implement it in your research.