Intact and Subunit LC-MS Characterization of mAbs and msAbs using Advanced Protein A Columns

See how innovative new ProA-MS methods can overcome traditional limitations in mAb and msAb characterization

Register Free: https://www.chromatographyonline.com/ca_w/protein-a

Event Overview:

Obtaining MS-based intact and subunit characterization of monoclonal antibodies (mAbs) and multispecifics (msAbs) is a crucial step in the development workflow. However, older analytical methods often fall short, lacking the depth of information required to support regulatory approval for today’s advanced therapeutics. This webinar introduces an innovative Protein A-MS pH-elution gradient method for achieving effective separations of protein variants with modulated binding affinities prior to direct intact high-resolution MS analysis. We will discuss how advanced ProA-MS methods can overcome traditional limitations and deliver the characterization needed to meet the evolving demands of advancing protein therapeutics.

Topics include:

• How to achieve in-depth characterization of mAbs and msAbs without prior sample preparation
• How new Protein A Affinity columns can provide valuable information beyond just titer
• How Protein A Affinity columns can be used in methods to offer additional characterization insights
• How to implement unique LC-MS analytical methods for both mAbs and msAbs

Speakers

Dr. Stephan Koza (Consulting Scientist, Waters Corporation)
Arnaud Delobel (Director, R&D and Innovation, Quality Assistance).

Register Free: https://www.chromatographyonline.com/ca_w/protein-a