Overcoming Compound Volatility with Sub-ambient ELSD

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E-Separation Solutions

E-Separation Solutions-10-10-2008, Volume 0, Issue 0

The success of evaporative light scattering detection relies on evaporating the eluent without destroying the analyte particle. Where the analyte is non-volatile, evaporator temperatures of 50-100 °C can be used without compound degradation, thus giving maximum sensitivity. However, when compounds have high vapor pressures and the eluent has a high boiling point (e.g. water), detection of semi-volatile compounds is problematic, because the evaporation temperature needs to be set at 30 °C or above in order to evaporate the solvent.