Feature|Videos|January 15, 2026

Spotlight on LC-MS Calibration: A Practical Guide for Bioanalysis

Key Takeaways

  • Matrix-matched multipoint ES with SILs remains the reference approach for quantitative LC-MS
  • Advancements in LC-MS and SIL availability have expanded acceptable calibration options.
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Dr. Joseph Lackey, Technical Manager at LGC Standards, provides an in-depth look at how calibration strategy selection in LC-MS bioanalysis influences data reliability.

Sponsored by LGC Standards

Accurate calibration strategy selection is central to reliable quantitative liquid chromatography-mass spectrometry (LC-MS) bioanalysis, influencing data quality, bias control, and regulatory compliance throughout the pharmaceutical lifecycle. Dr. Joseph Lackey, Technical Manager at LGC Standards, discusses a practical decision workflow with three case studies. The discussion compares matrix-matched external calibration, surrogate matrices, and single-point internal calibration using stable isotope-labeled standards, outlining both their strengths and limitations. Advances in LC-MS instrumentation and reference materials are enabling greater flexibility in calibration approaches, provided performance equivalence and scientific rationale are clearly established.

Read the Practical Guide Here

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