Implementation of Highly Efficient Protein Characterization and Preparative iCIEF
Whole column imaging detection capillary isoelectric focusing (iCIEF) has been recognized as a powerful tool for biopharmaceutical development and quality control. Unlike conventional single point detection capillary electrophoresis (CE) systems, in which light absorbance or emission at a specific point along the separation capillary is monitored, iCIEF detects a line of light that is passed through and radiates from the entire separation capillary. As a result, sequential snapshots of the whole separation capillary at different times are obtained. This allows sample separation and interaction in real time to be observed during electrophoresis, enabling fast analytical method development, high resolution separation, and high sample throughput.