Katherine K. Stenerson | Authors


Analysis of Terpenes in Cannabis Using Headspace Solid-Phase Microextraction and GC–MS

As the legalization of medicinal cannabis continues to sweep across the United States, an urgent need has developed for fast, accurate and efficient analytical testing. In addition to testing for contaminants and potency, there is also interest in the determination of terpene identity and concentration levels present in different strains of cannabis. Terpenes have been shown to have therapeutic uses for treatment of different medical conditions ranging from cancer and inflammation, to anxiety and sleeplessness. It is believed that the combination of terpenes and cannabinoids in cannabis produce a synergistic effect with regards to medical benefits. The traditional testing method for terpenes in plant materials involves a solvent-based extraction followed by GC analysis. In this work, headspace solid phase microextraction (HS-SPME) was used to identify and quantify terpene content in cannabis. The HS-SPME method provided several advantages over solvent extraction in that it provided a cleaner analysis, free of interferences from co-extracted matrix, and was non-destructive to the sample. A cannabis sample of unknown origin was first analyzed qualitatively by HS-SPME and GC-MS. Spectral library matching and retention indices were used to identify 42 different terpenes. Quantitative analysis was then performed for several selected terpenes using spiked samples. Method accuracy was >90%, with reproducibility of

Application of SPME Using an Overcoated PDMS–DVB Fiber to the Extraction of Pesticides From Spaghetti Sauce: Method Evaluation and Comparison to QuEChERS

A solid phase microextraction (SPME) method was developed using a new overcoated PDMS–DVB fiber for immersion extraction of pesticide residues from spaghetti sauce. The overcoating, which consisted of polydimethylsiloxane, offered protection to the SPME fiber, making it more resistant to physical and chemical damage. Also, it increased fiber selectivity for the smaller analyte molecules over macromolecules present in sample matrix. This then allowed it to be used in immersion extraction of a very complex matrix-spaghetti sauce. Performance of the overcoated fiber was compared to a nonovercoated fiber of the same chemistry for method accuracy, precision, and durability. The SPME method developed using the overcoated fiber was then compared to extraction of the same pesticides in spaghetti sauce using a conventional QuEChERS method for extraction and cleanup. SPME was comparable to the QuEChERs method for both accuracy and precision. However, its main advantage over QuEChERS was in sensitivity and method simplicity.