A discovery-based, untargeted metabolomics analysis of hundreds of yeast metabolites under robust, controlled extraction conditions followed by identification is described.
The metabolomics workflow described here combines untargeted (discovery) quadrupole time-of-flight (Q-TOF) liquid chromatography–mass spectrometry (LC–MS), targeted (confirmation) triple-quadrupole LC–MS-MS, and sophisticated data mining as an effective means to elucidate metabolite changes.