Wayne K. Way | Authors

Ascentis Express 5 micron columns provide a new choice for improving the performance of traditional HPLC systems. Based on Fused-Core particle technology, Ascentis Express provides the benefits of high speed and high efficiencies without the concerns of smaller particle columns. Due to the high efficiencies at low backpressures, Ascentis Express 5 micron can benefit conventional HPLC users with no drawbacks.

The Fused-Core particle consists of a 1.7 micron solid core and a 0.5 micron porous shell yielding a 2.7 micron diameter. One of the benefits of the Fused-Core particle is the small diffusion path (0.5 microns) compared to conventional fully porous particles. The shorter diffusion path minimizes peak broadening. In fact, there have been many reports on the vast improvements in efficiency provided by Fused-Core particles versus conventional particles. These improvements provide sub-2 micron like performance at half of the backpressure allowing Ascentis Express columns to be used in conventional HPLC as well as UHPLC systems.