LCGC Europe-04-01-2009

LCGC Europe

Event News

April 01, 2009

Departments

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Euroanalysis 2009, which is organized under the umbrella of the Division of Analytical Chemistry (DAC) of the European Association for Chemical and Molecular Sciences (EuCheMS), has become a premier event for scientists to discuss and hear presentations on a broad spectrum of analytical techniques from a diverse range of applications. Euroanalysis aims to bring together analytical chemists from various fields to stimulate communication and strengthen networks in analytical sciences.

BioFuels and Mass Spectrometry

April 01, 2009

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"...We will restore science to its rightful place, and wield technology's wonders to raise healthcare's quality and lower its cost. We will harness the sun and the winds and the soil to fuel our cars and run our factories...." - President Barack Obama, 44th President of the US, Inaugural address, 20 January 2009, Washington, DC.

Therapeutic Diazepam Monitoring in Human Plasma and Urine by HPLC: An Application for Alcoholism

April 01, 2009

Regular article

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A rapid and simple high performance liquid chromatography (HPLC) method with basic extraction assays was developed to investigate free diazepam levels in the plasma and urine samples of patients medicated with this drug for the management of alcohol withdrawal syndrome. The HPLC analysis was optimized and evaluated for linearity, imprecision, recovery, detection and quantification limits. The method showed linearity between 50–500 ng/mL (r2 ≥ 0.990). Coefficients of variations (%CV) were calculated to be in the range of 1.77–9.60. According to ICH guidelines, theoretical limits of detection (LOD) and quantification (LOQ) for plasma and urine were calculated as 8.3 ng/mL, 27.5 ng/mL and 8.2 ng/mL, 26 ng/mL respectively. Diazepam monitoring in plasma and urine displayed remarkable variations. The importance of adjusting doses according to individual requirements and the routine monitoring of plasma or urine for patients under medication is highlighted.