LCGC Europe-05-01-2020

In reversed-phase liquid chromatography, increasing the column temperature usually results in a decrease in analyte retention. Will this cause problems if a method does not call for control of the column temperature but the room temperature of the laboratory environment changes?

The dried blood spot (DBS) sampling technique has been around for decades, predominantly for small molecules and mainly in newborn screening. Although determination of proteins after DBS sampling is usually performed with immunometric assays, the combination with mass spectrometry (MS) is gaining interest. This article provides an overview of DBS sampling for mass spectrometry-based protein analysis. The first part will focus on clinical applications for DBSs and on sampling other biological matrices apart from whole blood, including dried matrix spots (DMSs). The second part will explore the new frontiers of the DBS sampling technology, including novel sampling materials/devices, and novel combinations with mass spectrometry. Examples of use in both qualitative and quantitative protein analysis are highlighted as well as examples using both bottom-up and top-down proteomics approaches.

“GC Connections” presents the column’s annual review of new developments in the field of gas chromatography seen at Pittcon and other venues in the past 12 months.

One of the biggest failures with purchasing chromatograph systems and chromatography data system (CDS) software is either the total lack of or poorly written user requirements. So, how can you write acceptable requirements? Is specifying a chromatograph the same as software?

This yearly report on new products introduced in the preceding year, since May 2019, covers sample preparation instrumentation, supplies, and accessories.