A Novel Sample Preperation Approach to Increase the Throughput of Pesticide Analysis by LC–MS–MS
November 01, 2009
The high-throughput analysis of pesticides in raw agricultural commodities has been hindered by the slow and laborious sample preparation stages. Using traditional clean-up procedures, as many as 20 steps are required to get the sample in a convenient form for analysis, which typically limits sample throughput to eight samples per day. This study evaluates a new approach using a novel laboratory mill and pulverizer to extract various pesticide residues from different kinds of plant materials and to identify and quantify the active ingredients by liquid chromatography tandem mass spectrometry (LC–MS–MS). It shows that by automating the procedure, the number of clean-up steps can be reduced significantly, which increases sample throughput over traditional approaches.