Capillary gel electrophoresis (CGE) is a high-resolution, label-free technique for the quality control of mRNA-based therapies. Because the poly(A) tail plays a critical role in mRNA stability and translation efficiency, accurately detecting and quantifying tailless species is essential to ensuring therapeutic efficacy and patient safety. This article explores how to optimize the SCIEX BioPhase™ 8800 system and RNA 9000 Purity & Integrity Kit to achieve the resolution required for today’s advanced mRNA medicines. Key method parameters, including electric field strength, capillary temperature, and sample concentration, are examined to support reliable separation and impurity detection.
In this seventh part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor explains why 2.1-mm i.d. columns are less preferred for pharmaceutical quality control.
Ultrahigh-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF-MS) shows how soaking and cooking break down soybean fats.
Nicolai Zederkopff explores chromatography’s role to help implement the maximum tolerable level (MTL) framework for food adulteration to translate analytical results into compliance decisions.
Franz Berthiller (BOKU University, Austria) discusses a multilaboratory study that used retention index markers to align mycotoxin liquid chromatography–mass spectrometry (LC–MS) data.
A QuEChERS/ultrahigh-performance liquid chromatography and high-resolution mass spectrometry (UHPLC-HRMS) method tracks 23 plant toxins in cereals and spices. Michele Suman discusses the method with LCGC International.
