Adapting high-performance liquid chromatography (HPLC) methods to the capillary scale opens new possibilities for pharmaceutical analysis and sustainability. This paper, featuring a collaboration with the University of Pavia and Axcend, examines real-world method conversions that preserve performance while minimizing waste. Case studies include detection of a polar impurity in pediatric ibuprofen mini-tablets using a micro-HPLC-UV method, and a USP <621> guided adaptation for hydrochlorothiazide demonstrating over 99% solvent reduction. Practical guidelines, expected trade-offs, and data from both academic and regulated environments will offer a roadmap to adapting validated pharmaceutical monographs to the capillary scale without the need for revalidation.
In this seventh part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor explains why 2.1-mm i.d. columns are less preferred for pharmaceutical quality control.
Ultrahigh-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF-MS) shows how soaking and cooking break down soybean fats.
Nicolai Zederkopff explores chromatography’s role to help implement the maximum tolerable level (MTL) framework for food adulteration to translate analytical results into compliance decisions.
Franz Berthiller (BOKU University, Austria) discusses a multilaboratory study that used retention index markers to align mycotoxin liquid chromatography–mass spectrometry (LC–MS) data.
A QuEChERS/ultrahigh-performance liquid chromatography and high-resolution mass spectrometry (UHPLC-HRMS) method tracks 23 plant toxins in cereals and spices. Michele Suman discusses the method with LCGC International.
