The Analysis of Fluorescent Whitening Agents Using Reversed-Phase HPLC and Mass Spectrometry

Here's a taster of what to look forward to at the 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025), which will be held from Sunday through Thursday, 15–19 June, 2025, in Bruges, Belgium.

This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.

This article discusses several practical examples using bioinert columns, demonstrating their ability to deliver reliable, high-resolution results with precision and reproducibility.

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A two-step workflow to extract pure mass spectra from comprehensive two-dimensional liquid chromatography high-resolution mass spectrometry (LC×LC–HRMS) data has been created and tested.