
Gas chromatography (GC) tests show spoilage yeasts alter flavor and raise alcohol in NoLo beer.
This work presents an Automated Sample Preparation procedure for MOSH/MOAH analysis of Seasoning Oils. We compare results from a manual epoxidation procedure compliant with DIN 16995 with results based on fully automated sample preparation (epoxidation and saponification) compliant with ISO 20122. In both cases, online clean-up via activated aluminum oxide (AlOx) are used to remove interfering n-alkanes from the MOSH fraction during the HPLC run. Automated data evaluation using a dedicated software (GERSTEL ChroMOH) is presented.





