Bioanalytical Separations and Mass Spectrometry Methods

A Thursday afternoon session entitled, “Bioanalytical Separations and Mass Spectrometry Methods,” will be held in Room W179B, beginning at 1:30 pm­, presided over by Honglan Shi of Missouri University of Science and Technology.

Kevin Clark of the Beckman Institute for Advanced Science and Technology will kick off the session with a talk on microextraction approaches for profiling neuronal RNA modifications by mass spectrometry (MS).

Next, at 1:50 pm, Michael Rerick of the University of Pittsburgh will present a paper entitled “ In Vivo Study of Ectopeptidases Using Electroosmotic Perfusion-Microdialysis Coupled with LC-MS.”

A talk on the characterization of transport of drug analogs through membrane mimics with surface plasmon resonance (SPR) spectroscopy and MALDI-MS will be presented next, by co-authors Daniel Stuart of the University of California, Riverside. This talk starts at 2:10 pm.

At 3:05 pm, co-authors Amarakoon Appuhamilage Thilini Navodi Amarakoon of the University of Arizona, will present work on “Quantification of Intracellular HNO Delivery using a Cell-permeant, Thiol-functionalized Fluorescein with CZE.” Thispresentation begins at 2:30 pm.

The next talk is entitled, “Quantifying Cell Number for Metabolomic Analysis using Calcein Blue Dye as an Internal Standard via LC-UV-MS,” and will be presented by Katherine Sandersof Saint Louis University, starting at 3:05 pm.

“Polymer Brush on Silica for RPLC-MS of Intact IgG without use of Trifluoroacetic Acid” will be presented next, by Yun Yang of Purdue University. This presentation starts at 3:25 pm.

At 3:45 pm, Lindsay of West Virginia University will present work on analytical separations to evaluate the N-glycan changes on an antibody therapeutic that guide potency. The presentation begins at 3:45 pm.

In the final talk of this session, Mohammadmehdi Azizi of the University of Texas at Arlington will present “Extraction, Fractionation and Enrichment of Membrane Proteins in Yeast Proteome by Fluoroalcohol Two-phase Systems.” The presentation begins at 4:05 pm and concludes at 4:25 pm.