CE-TOFMS Analysis of Amino Acids
Researchers from Utrecht University?s (Utrecht, The Netherlands) Department of Biomedical Analysis have described a capillary electrophoresis?time-of-flight mass spectrometry (CE?TOFMS) method for analyzing amino acids in the urine of patients with urinary tract infections.
Researchers from Utrecht University's (Utrecht, The Netherlands) Department of Biomedical Analysis have described a capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) method for analyzing amino acids in the urine of patients with urinary tract infections. The method involves the use of capillaries coated with polybrene and poly(vinyl sulfonate) to obtain migration time repeatability with relative standard deviations of less than 2%. Limits of detection for the amino acids were reported to be as low as 20 nM. According to the researchers, the method shows good potential for the screening of body fluids based on the analysis of endogenous low molecular weight metabolites such as amino acids and related compounds.
Modern HPLC Strategies: Improving Retention and Peak Shape for Basic Analytes
August 16th 2024In high-performance liquid chromatography (HPLC), it is common for bases and unreacted ionized silanols on silica-based columns to cause irreproducible retention, broad peaks, and peak tailing when working with basic analytes. David S. Bell, Lead Consultant at ASKkPrime LLC offers innovative HPLC strategies that can help mitigate such issues.
Reliable and Objective Antibody Analysis with the New Agilent ProteoAnalyzer System
September 18th 2024This technical overview compares the characterization of NISTmAb using the ProteoAnalyzer with published NIST data obtained via traditional single capillary CE-SDS technology. The results show strong correlation with the reference data, confirming that the ProteoAnalyzer is a reliable tool for analytical workflows.
