CF3-DLS for the Analysis of Drug Delivery Particles

This technical note demonstrates the SEC-based separation and quantification of Cas9 IVT mRNA (with a total length of approximately 4500 nucleotides) and its aggregates.

SEC data demonstrates mRNA aggregate analysis for vaccine stability. Optimal separation occurs at 100 mM NaCl and low flow rates, enhancing mRNA production quality.

The data show that a separation between circular and linear plasmid DNA is possible and the performed linearization can be monitored using SEC-HPLC.

This application showcases the utility of SEC to resolve s.s. oligonucleotides from duplexes in mixtures of four oligonucleotide lengths (21, 40, 80, and 100mers).

Experience high recoveries from the first injection! YMC Accura BioPro IEX SF columns provide excellent peak shapes for the ion-exchange analysis of mAbs.

The optimized LC-MS/MS method covering 388 signaling lipids shows that the bioinert coated YMC Accura Triart C18 column combines high sensitivity with high resolution.