A multinational research team developed a green, miniaturized CPME-HPLC-DAD method to simultaneously extract and detect four tetracyclines in human urine, offering a simpler, cheaper alternative to LC-MS/MS with improved sensitivity. LCGC International spoke to Fedra Vasileiou, Victoria Samanidou, and Abuzar Kabir, three of the authors of the paper that resulted from this work.
Tetracyclines are widely used antibiotics that fight a broad range of bacteria, commonly treating urinary tract infections, acne, respiratory infections, and skin infections. Since the first one was discovered decades ago, they have evolved into three "generations," with tetracycline, oxytetracycline, doxycycline, and chlortetracycline now most common.
