Developing Better HPLC(MS) Methods

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Webinar Date/Time: Thursday, December 14th, 2023 at 8am PST | 11am EST | 4pm GMT | 5pm CET

Join us as we delve into the essential guide for setting specifications, optimizing chromatographic parameters, and mastering mobile phase design to achieve robust and reproducible results. Don't miss out on practical tips for sample preparation and detector settings that will empower you to develop HPLC methods with unwavering consistency.

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Event Overview:

Where do you start when you want to develop a better HPLC method? Whether you work in a regulated environment or not, setting specifications for your HPLC protocols is good practice. This essential guide sets out guidelines for chromatographic parameters such as retention, resolution, and efficiency that can be used to improve HPLC methods prior to method validation. It will explain how mobile phase design and instrument parameters can be used to achieve improvements in method robustness and what to look for when a method is failing. Practical tips for sample and eluent preparation, and the correct detector settings to use will also be discussed. We will look at the major factors that affect chromatographic robustness to give you the tools to develop better methods that will give you consistent, reproducible chromatography.

Topics Covered:

  • What target specifications should be set for an HPLC method.
  • How sample preparation can impact the sensitivity and robustness of an HPLC method.
  • Stationary phase chemistry and important column properties.
  • Designing smart eluent solutions.
  • Detector parameters to improve quality and robustness.
  • Developing inherently robust methods.
  • Aspects of LC-MS methods which can (and should) be optimized.


Kevin Schug
Professor and the Shimadzu Distinguished Professor of Analytical Chemistry, Department of Chemistry and Biochemistry
The University of Texas at Arlington

Kevin A. Schug is Professor and the Shimadzu Distinguished Professor of Analytical Chemistry in the Department of Chemistry and Biochemistry at The University of Texas at Arlington (UTA). He is also Director of the Collaborative Laboratories for Environmental Analysis and Remediation (CLEAR) at UTA. He received his B.S. degree in Chemistry in 1998 from the College of William and Mary, and his Ph.D. degree in Chemistry from Virginia Tech in 2002 under the direction of Prof. Harold M. McNair. From 2003-2005, he performed post-doctoral research at the University of Vienna in Austria with Prof. Dr. Wolfgang Lindner.

Since joining UTA in 2005, his research has been focused on the theory and application of separation science and mass spectrometry for solving a variety of analytical and physical chemistry problems, in the fields of environmental, pharmaceutical, biological, and energy research. He has over 200 peer-reviewed publications and over 600 presentations, posters, and invited talks to his group’s credit. He has been the primary mentor and research advisor to more than 30 graduate and 80 undergraduate students. Dr. Schug has received several research awards, including the 2009 Emerging Leader Award in Chromatography by LCGC Magazine and the 2013 American Chemical Society Division of Analytical Chemistry Young Investigator in Separation Science Award. He was named to the 2019 and the 2021 The Analytical Scientist’s Top 100 Power List of the most influential analytical chemists in the world. For his teaching, he received the 2014 University of Texas System Regents’ Outstanding Teaching Award and in 2017, was awarded the J. Calvin Giddings Award for Excellence in Analytical Chemistry Education by the American Chemical Society. He is a Fellow of both the University of Texas System’s and U.T. Arlington’s Academy of Distinguished Teachers.

Dr. Dawn Watson
CHROMacademy Product Manager

Dawn Watson completed her Ph.D. in synthetic inorganic chemistry at the University of Strathclyde, Glasgow, followed by postdoctoral research on small molecule reaction kinetics at Princeton University. Following several years working for a major instrument manufacturer, she became a technical expert for CHROMacademy in 2013. She has expertise in various analytical techniques, including HPLC, GC, GC–MS, LC–MS, NMR, and molecular spectroscopy, as well as numerous wet chemistry and sample preparation techniques.

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