Ep. 42: Did You Look at the Raw Data?

Welcome to “Analytically Speaking,” the podcast from LCGC International and Spectroscopy.

In this episode, podcast co-hosts Dr. Dwight Stoll and Dr. James Grinias talk with Professor Kelly Hines. Dr. Hines is an associate professor in the Department of Chemistry at the University of Georgia in Athens. She received her B.S. degree in Chemistry from the University of Florida, and then completed the Ph.D. in Chemistry at Vanderbilt University. Her doctoral studies focused on monitoring biomolecular signatures of disease via ion mobility and mass spectrometry techniques, and then she moved on to post-doctoral experiences at two different institutions. The first stop was at the metabolomics resource core at the Mayo Clinic, and the second stop was in the Department of Medicinal Chemistry at the University of Washington School of Pharmacy. Kelly has won several awards in the field of chemical analysis, including recognition as a Female Role Model in Analytical Chemistry by Analytical and Bioanalytical Chemistry, as an Emerging Investigator by the Journal of the American Society for Mass Spectrometry, an ASMS Research Award (which is one of the top honors that young investigators in MS can receive), and very recently was named as the Chemist of the Year by the Northeast Georgia Section of the American Chemical Society.

In a wide-ranging conversation, we discuss the early origins of Kelly’s interest in science rooted in her opportunity to explore the plant nursery managed by her father, and then her turn from civil engineering to analytical chemistry inspired by an early undergraduate course “Chemistry for Engineers”. We discuss Hines’ recent and ongoing work involving the use of ion mobility mass spectrometry (IMS-MS), with pre-separation using chromatography when appropriate, for multi-omics studies, aimed at deeper understanding of the biochemistry of organisms at the metabolite level. We also discuss Kelly’s positive experiences developing peer networks through regular attendance at conferences, particularly the American Society for Mass Spectrometry (ASMS) annual meeting, experiences in her second post-doctoral position that inspired her to pursue an academic position, and her approach to mentoring graduate students who show up in her laboratory with diverse backgrounds, interests, and abilities.

Literature Discussed:

1. J.M. Carpenter, H.M. Hynds, K. Bimpeh, K.M. Hines, HILIC-IM-MS for Simultaneous Lipid and Metabolite Profiling of Bacteria, ACS Meas. Sci. Au 4 (2024) 104–116.https://doi.org/10.1021/acsmeasuresciau.3c00051.
2. H.M. Hynds, J.M. Carpenter, K.M. Hines, Rapid Multi-Omics for Bacterial Identification Using Flow Injection–Ion Mobility–Mass Spectrometry, Anal. Chem. 97 (2025) 13809–13816.https://doi.org/10.1021/acs.analchem.5c00417.
3. H.M. Hynds, K.M. Hines, Ion Mobility Shift Reagents for Lipid Double Bonds Based on Paternò–Büchi Photoderivatization with Halogenated Acetophenones, J. Am. Soc. Mass Spectrom. 33 (2022) 1982–1989.https://doi.org/10.1021/jasms.2c00211.

More about our hosts:

Dwight Stoll, PhD:

Dwight R. Stoll is a professor of chemistry at Gustavus Adolphus College in St. Peter, Minnesota. He received his PhD from the University of Minnesota, under Professor Peter Carr, working on the development of fast, comprehensive two-dimensional liquid chromatography (2D-LC). Stoll’s current primary research focus is on the development of 2D-LC for both targeted and untargeted analyses. Active research projects in his laboratory touch on most aspects of multidimensional separation methodologies, including optimization strategies, characterization of selectivity in reversed-phase LC, instrument development, and applications in biopharmaceutical analysis. Stoll is the author or co-author of more than 80 peer-reviewed publications and six book chapters and has instructed numerous short courses in 2D-LC. In 2011 he was the recipient of LCGC’s Emerging Leader in Chromatography Award. In 2017 he received the Georges Guiochon Faculty Fellowship, and was recognized with an Agilent Technologies Thought Leader Award. He is also a member of LCGC’s editorial advisory board and is the editor of the “LC Troubleshooting” column in LCGC.

Jerome Workman, Jr., PhD:

Jerome (Jerry) J. Workman, Jr. is the Executive Editor for LCGC and Spectroscopy. He has held positions as CTO, executive VP, senior research fellow, director, and senior scientist at companies of all sizes, from start-ups to world-leading corporations. He has been an adjunct faculty member of four universities and advised multiple graduate students. He has more than 75 U.S. and international patent applications and 30 issued U.S. and international patents and multiple trade secrets, as well as 500+ technical publications, and 20 reference book volumes on a broad range of spectroscopy and data processing techniques. He has received multiple awards from scientific societies, and has taught annual courses in spectroscopy, chemometrics, and statistics for the AOAC, ACS, ISA, FACSS, and at several universities and corporations. He is a Fellow of the American Institute of Chemists (FAIC), the American Society for Testing and Materials (ASTM), and the Royal Society of Chemistry in the UK (FRSC, CChem, CSci). Jerry holds B.A and M.A degrees from Saint Mary's University of Minnesota, and a PhD degree from Columbia Pacific University working in near-infrared spectroscopy. He is an alumnus of both Columbia University Business School and the MIT Sloan School of Management.

About the Analytically Speaking Podcast:

Analytically Speaking, the podcast from LCGC and Spectroscopy, addresses important issues in separation science and analytical spectroscopy. Topics include new analytical techniques, methods, and approaches; the latest trends; advances in instrument and software technology; practical solutions for specific applications; recent papers in the scientific literature and their applicability; challenges and solutions for data analysis and interpretation; analytical chemistry theory and fundamentals (from advanced research to tutorials and troubleshooting); and more. Our regular hosts are Dwight Stoll, PhD, a professor of chemistry at Gustavus Adolphus College in St. Peter, Minnesota, and Jerry Workman, PhD, a spectroscopist, noted author, and currently the Senior Technical Editor of Spectroscopy and LCGC. Dwight covers separation science and Jerry addresses spectroscopy related topics.

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