Advances in DNA/RNA chromatography columns led to stable, pure mRNA production, supporting the development of COVID-19 vaccines rapidly.
News|Articles|October 9, 2025
Examining Column Selectivity Differences Across Various L1 Designation Columns for Paracetamol EP Impurities Monograph (Oct 2025)
Author(s)Waters Corporation
he United States Pharmacopeia (USP) has designations for all columns stationary phases used in the monograph methods. These designations outline the stationary phase type, i.e. fully porous or solid-core, and any ligand attachments, i.e. C18 or Phenyl to be used.1 However, beyond that no column specifics are given. With a multitude of columns that fit into the different designations, understanding that not all columns are the same is vital when selecting a stationary phase for a monograph method. This application note examines three columns that all fit into the L1 designation when analyzing paracetamol impurities. Selectivity differences between the columns are considered in relation to the impurities.
Related to this article
Steven Ray Wilson's AFFL LC–MS system runs thousands of injections per column, but he calls validation and standardization the field's real barrier.
Kinjal Bhatt and Ina Varfaj describe imposter syndrome fading through repeated exposure and conference talk rather than a single breakthrough moment.
Mass spectrometry can teach core chemistry concepts beyond technique training or product characterization, though adoption barriers remain.
Automated sequential tube sampling and chemometrics supplement GC–MS, adding time profiling and fast product-line comparison for real-world insight.
Ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) tracks blood metabolite changes after repetitive transcranial magnetic stimulation (rTMS)-cognitive training therapy.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.
SFC Europe 2026 brings global experts to Basel for courses, scientific talks, workshops, and insights into greener separation technologies.