Getting the Best Out of Solid-Core Technology for Small- and Large-Molecule Analysis



Wednesday, June 9, 2021 at 1pm EDT | 12pm CDT | 10am PDT

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Event Overview:

The growth of solid-core particle technology in liquid chromatography has seen an increase in the applicability of these types of particles to a wider range of workflow solutions. Initial offerings were primarily targeted towards small-molecule separations. More recently, however, wide pore solid core particles have been released onto the market, designed specifically for the separation of large biomolecules. Additionally, there has also been an increase in the diversity of stationary phases available to the separation scientist. This presentation will begin by reviewing some of the fundamental benefits of solid core technology, before quickly moving onto how to best utilize the new technology.

The use of different pore sizes and pore volumes and the impact this has on separation performance, as well as the column loading capacity, will be discussed. This will be done for a range of different molecular mass molecules and the impact of pore size and pore volume will be demonstrated, with a particular focus on the separations of biomolecules.

Example separations will include intact and partially digested mAbs, as well as a full tryptic digest. The protocols for the digestion will be discussed and the impact of different column chemistries will be investigated to demonstrate the importance of assessing a range of column chemistries during method development, even for large biomolecule separations.

The final part of the presentation will look at some more unusual aspects that can occur in the field of chromatography, such as the influence of flow rate on retention factor. It might be considered that the flow rate does not have an impact on the relative retention time of an analyte, but this is not always the case. This will be discussed in the context of method transfer, along with factors such as equilibration time with specific reference to solid core materials.

Key Learning Objectives:

  • Learn the importance of pore size for biomolecular separations
  • Learn the fundamental benefits of solid-core technology
  • Review examples of large- and small-molecule separations for solid-core method development

Who Should Attend?

  • Researchers involved in method development from all industries

For technical questions please contact Martha Devia:


Matt James, Ph.D.
Senior Research Scientist

Matt James is a Senior Research Scientist at Avantor Sciences. Matt gained his PhD at University of Bristol, working on the analysis of archaeological samples and also worked within the pharmaceutical industry for a year. Over the last 11 years, he has worked within the Technical Support, Applications and R&D teams within Hichrom, now part of Avantor. Recent focuses have been HPLC/UHPLC method transfer and translation, the use of solid core phases, development of next generation LC stationary phases and the development of LC methods for key applications, such as the analysis of nitrosamines by LC-MS/MS. Matt has co-authored seven peer reviewed articles and presented work at several conferences.

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