HILIC with Alcohol as the Organic Modifier
Researchers from Amgen (Thousand Oaks, California) examined the separation mechanism in the analysis of polar and basic hydrazines using hydrophilic interaction liquid chromatography (HILIC) with alcohol rather than acetonitrile as a weak eluent.
Researchers from Amgen (Thousand Oaks, California) examined the separation mechanism in the analysis of polar and basic hydrazines using hydrophilic interaction liquid chromatography (HILIC) with alcohol rather than acetonitrile as a weak eluent. They studied separation parameters such as alcohol type and concentration, acid modifier, buffer in the mobile phase, stationary phase type, and column temperature. They found that both electrostatic and hydrophilic interactions contributed to the retention and separation of the hydrazines. The researchers coupled the optimized HILIC technique with chemiluminescent nitrogen detection for the hydrazine analysis.
The 26th Norwegian Symposium on Chromatography
March 29th 2024The 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.
Adjustable Polyacrylamide Stationary Phases Enhance Separation Performance in Liquid Chromatography
June 12th 2023Adjustable polyacrylamide stationary phases developed by researchers enhance separation performance in liquid chromatography, according to a recent publication. The study demonstrates the synthesis of vinyl modified silica and the successful preparation of polyacrylamide modified silica (PAM-SIL) stationary phases, allowing for fine control of separation performance.
An Interview with AI About Its Potential Role in Separation Science
May 17th 2023We interviewed an AI program (ChatGPT) for LCGC North America asking questions about AI and its role in various applications for separation science to include data analysis, and high performance liquid chromatography (HPLC), hydrophilic-interaction chromatography (HILIC), reversed-phase liquid chromatography (RPLC), liquid chromatography–mass spectrometry (LC–MS), gas chromatography–mass spectrometry (GC–MS), high resolution mass spectrometry (HRIM–MS), high resolution tandem mass spectrometry (HRMS/MS), and related topics.
