How to Troubleshoot HPLC Extra Peaks and Contamination Like a Pro



Thu, Aug 18, 2022 11:00 AM EDT How To Professionally Troubleshoot Extra Peaks and Contamination in HPLC.

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Key learning objectives

Extra peaks resulting from contamination is our most asked about problem. Contamination can be introduced into the HPLC system at any point of the process, making it an incredibly complex and time-consuming issue to troubleshoot. Previously we have discussed the types and sources of contamination. Now it’s time for us to walk you through a strategy to troubleshoot this problem quickly and effectively using simple experiments to narrow down the source of the problem.

Topics covered:

  • Types and sources of contamination
  • How to clearly define the problem and what it tells us
  • Initial experiments to rule out the instrument
  • Further investigational experiments

Dr. Dawn Watson
CHROMacademy Product Manager
Crawford Scientific

Dawn completed her PhD in synthetic inorganic chemistry at the University of Strathclyde, Glasgow, followed by postdoctoral research on small molecule reaction kinetics at Princeton University. Following several years working for a major instrument manufacturer, she became a technical expert for CHROMacademy in 2013. She has expertise in various analytical techniques, including HPLC, GC, GC–MS, LC–MS, NMR, and molecular spectroscopy. As well as numerous wet chemistry and sample preparation techniques.

Colin Towers
Senior Technical Support Chemist
Crawford Scientific

After completing a degree in pharmacy, Colin Towers pursued a career in analytical chemistry. Following three years performing routine testing and three years instrument maintenance and calibration, he moved into a role as a method development and validation specialist in contract research. He gained 11 years of experience in developing methods for HPLC, LC–MS, GC, GC–MS and SPE in a high-throughput commercial environment, followed by three years lecturing in forensic analytical toxicology and a further three years working as a community pharmacist. Colin has now been with Crawford Scientific for 10 years as a technical specialist, consultant, and trainer.

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