Identification, Mapping and Relative Quantitation of SARS-CoV-2 Spike Glycopeptides by Mass-Retention Time Fingerprinting

November 12, 2020
Agilent Technologies, Inc.

Webcast

***Live: North America: Wednesday, December 2, 2020 at 11am EST | 10 CST | 8am PST Asia Pacific: Wednesday, December 2, 2020 at 8:30am IST | 11am CST | 12pm JST Europe: Wednesday, December 2, 2020 at 2pm GMT | 3pm CST *** Slaying a Glyco-Monster: Rod Chalk describes a novel and accessible LC/MS approach to characterization of SARS-CoV-2 spike protein glycans.***On demand available after final airing until Dec. 2, 2021***

Register Free:https://www.chromatographyonline.com/lcgc_w/glycopeptides

Event Overview: The technical issues surrounding the characterization of SARS-CoV-2 spike (S) protein glycosylation and its importance for quality control of expressed spike protein will be discussed. A novel approach based on reversed phase LC-MS/MS with a Q-TOF will be described capable of identifying hundreds of glycopeptides which were used to populate a mass-retention time database. Participants will learn how this database can be incorporated into a simple, powerful, rapid and robust characterization workflow.

  • Challenges of SARS-CoV2 spike protein glycan analysis
  • Novel glycopeptide discovery workflow
  • SARS-CoV2 spike glycopeptide database
  • SARS-CoV2 spike Mass-Retention Time Fingerprinting (MRTF) workflow

Key Learning Objectives:

  • Understanding SARS-CoV-2 spike protein glycan structure
  • Novel approaches to glycopeptide separation & analysis
  • Conceptual understanding of the mass-retention time fingerprinting technique

Speaker: Dr. Rod Chalk, Head of Mass Spectrometry, New Centre for Medicines Discovery, Oxford University

Times and Dates: North America: Wednesday, December 2, 2020 at 11am EST | 10am CST | 8am PST

Asia Pacific: Wednesday, December 2, 2020 at 8:30am IST | 11am CST | 12pm JST

Europe: Wednesday, December 2, 2020 at 2pm GMT | 3pm CST

On Demand until Dec. 2, 2021.

Sponsor: Agilent

Register free:​ ​ ​ ​ ​ ​https://www.chromatographyonline.com/lcgc_w/glycopeptides ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​