News|Articles|November 3, 2025

Improving Sample Throughput of HPLC Methods

Author(s)Waters

Improve validated HPLC methods by switching from fully porous to CORTECS™ Premier C18 5 µm Columns to boost throughput, cut solvent use, and enhance method greenness.


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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.
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