LC-MS Used in Simultaneous Quantification of Three Iridoids in Rat Plasma

October 28, 2011

Scientists Zhi-Min Long, Kai-Shun Bi, Yan-Shuang Huo, Xiao-Yu Yan, Xu Zhao, and Xiao-Hui Chen at Shenyang Pharmaceutical University, (Shenyang, China) developed and validated a sensitive and efficient liquid chromatography-mass spectrometry (LC-MS) method for the simultaneous determination of geniposide 6a-hydroxygeniposide, and genipin gentiobioside in rat plasma.

Scientists Zhi-Min Long, Kai-Shun Bi, Yan-Shuang Huo, Xiao-Yu Yan, Xu Zhao, and Xiao-Hui Chen at Shenyang Pharmaceutical University, (Shenyang, China) developed and validated a sensitive and efficient liquid chromatography-mass spectrometry (LC-MS) method for the simultaneous determination of geniposide 6α-hydroxygeniposide, and genipin gentiobioside in rat plasma.

After the addition of internal standard salidroside and acidification (formic acid, 0.1%), plasma samples were carried out by protein precipitation with acetonitrile and separated on a C18 column (200 mm×4.6 mm, 5 μm) within a runtime of 15.0 min. The linear ranges were 2–250 ng/mL for both 6α-hydroxygeniposide and genipin gentiobioside and 2–2000 ng/mL for geniposide, respectively.

The validated method was successfully applied to a pharmacokinetics study of geniposide, 6α-hydroxygeniposide, and genipin gentiobioside in rats after oral administration of a Zhi-zi-chi (gardenia and prepared soybean) decoction.

The abstract was published in the October 2011 issue of Journal of Separation Science, Volume 34, pp 2854-2860.

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