LC-MS Used in Simultaneous Quantification of Three Iridoids in Rat Plasma
Scientists Zhi-Min Long, Kai-Shun Bi, Yan-Shuang Huo, Xiao-Yu Yan, Xu Zhao, and Xiao-Hui Chen at Shenyang Pharmaceutical University, (Shenyang, China) developed and validated a sensitive and efficient liquid chromatography-mass spectrometry (LC-MS) method for the simultaneous determination of geniposide 6a-hydroxygeniposide, and genipin gentiobioside in rat plasma.
Scientists Zhi-Min Long, Kai-Shun Bi, Yan-Shuang Huo, Xiao-Yu Yan, Xu Zhao, and Xiao-Hui Chen at Shenyang Pharmaceutical University, (Shenyang, China) developed and validated a sensitive and efficient liquid chromatography-mass spectrometry (LC-MS) method for the simultaneous determination of geniposide 6α-hydroxygeniposide, and genipin gentiobioside in rat plasma.
After the addition of internal standard salidroside and acidification (formic acid, 0.1%), plasma samples were carried out by protein precipitation with acetonitrile and separated on a C18 column (200 mm×4.6 mm, 5 μm) within a runtime of 15.0 min. The linear ranges were 2–250 ng/mL for both 6α-hydroxygeniposide and genipin gentiobioside and 2–2000 ng/mL for geniposide, respectively.
The validated method was successfully applied to a pharmacokinetics study of geniposide, 6α-hydroxygeniposide, and genipin gentiobioside in rats after oral administration of a Zhi-zi-chi (gardenia and prepared soybean) decoction.
The abstract was published in the October 2011 issue of Journal of Separation Science, Volume 34, pp 2854-2860.
Identifying and Rectifying the Misuse of Retention Indices in GC
December 10th 2024LCGC International spoke to Phil Marriott and Humberto Bizzo about a recent paper they published identifying the incorrect use of retention indices in gas chromatography and how this problem can be rectified in practice.
Overcoming Common Challenges to Determine Residual Impurities Using IC in APIs with Limited Water
December 10th 2024Organic solvents are generally not compatible with ion chromatography (IC) systems. The approach presented here assists the use of organic solvents for sample preparation and provides a mechanism for the removal of the organic solvents from the chromatographic flow path.
