Minimizing Fluid Loss by Monitoring Back Pressure during Clarification

Peristaltic pumps are essential in biologics manufacturing but are prone to rupture or leaks when back pressure rises beyond design limits, leading to costly fluid loss and safety risks. This case study describes how a biopharma CDMO experienced a tubing rupture during lentivirus clarification, resulting in product loss, additional quality testing, and facility decontamination. The solution required minimal changes, offered straightforward validation, and provided robust process control.