Sample Prep Protocol Developed for Neuropeptide Research

August 7, 2007

In a recent article published in the Journal of Proteomic Research, a group of researchers reported a new two-dimensional liquid chromatography (2D LC) protocol for the preparation and analysis of rat neuropeptides.

In a recent article published in the Journal of Proteomic Research, a group of researchers reported a new two-dimensional liquid chromatography (2D LC) protocol for the preparation and analysis of rat neuropeptides.

As opposed to the more common method used to prepare samples in this area, which utilizes microwave irradiation to inactivate mammalian proteases, the protocol developed here utilized boiling water, which stops the enzymes responsible for nonspecific protein degradation. This technique produced results similar to those obtained with the microwave protocols, but was easier to implement and involved standard laboratory equipment.

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