Simplifying Sample Preparation

This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.

Hydrophobic deep eutectic solvents have proven useful in extracting non-polar target compounds, though researchers have shown there is room for further development.

Aflatoxins are a family of toxins made by certain fungi that are found on agricultural crops, such as maize (corn), peanuts, and cottonseed.

Nadia Gadi and Allisson Barros de Souza spoke to LCGC International about their investigation into the application of electrochemical advanced oxidation processes (eAOPs) for the efficient removal of pharmaceutical residues in wastewater.

A study has compared solid-phase extraction (SPE) to traditional normal-phase liquid column chromatography (LCC) methods for the analysis of hydrocarbons by gas chromatography–mass spectrometry (GC–MS).

In this month's edition of the LCGC Blog, Emanuela Gionfriddo discusses chromatography resources that can help make the field more accessible to newcomers and veterans alike.