LCGC UST
Press Release|Articles|June 17, 2025

Smart-Subtract™ – Software for comparing complex GC–MS chromatograms

Smart-Subtract™ is a specialist software tool for GC–MS data analysis that highlights differences between two chromatograms by subtracting one from the other. Enabling rapid detection of trace-level compounds and subtle chemical variations in complex mixtures, Smart Subtract helps analysts focus on what’s new or changing without the need for time-consuming manual work.


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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.