Top Tips to Improve Reproducibility and Sensitivity in Solid Phase Extraction

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Webinar Date/Time: Thursday, May 18th, 2023 at 8am PDT | 11am EDT | 4pm BST | 5 pm CEST

Solid Phase Extraction (SPE) is a powerful sample preparation tool but is poorly understood and rarely optimized. As with all chromatographic techniques, a reproducible method is of great importance. Therefore, in this webcast, we investigate the parameters that affect SPE reproducibility and present tips and tricks to ensure the lowest possible variability in sample recovery.

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Event Overview:

A robust and sensitive method is the product of meticulous method development. In this webcast, we will consider the most pertinent steps in SPE method development and how the decisions that are made can impact on reproducibility and sensitivity. We will also highlight best practices for ensuring optimum analyte recovery (sensitivity) and explain how to obtain lower detection limits even with generic SPE protocols.

Topics Covered:

Essential SPE method optimization

  • Sorbent selection: the key to good reproducibility and sensitivity
  • Understanding your extraction chemistry
  • Tips for optimizing SPE protocol steps
  • Sorbent equilibration and sample loading considerations
  • Solvent flows, soak steps and drying steps: expert tips
  • Wash steps: how to avoid the hazards and optimize your recovery
  • Sample elution: be brave to achieve lower detection limits
  • Matrix effects and how to overcome them


Dr. Doug Raynie
Associate Research Professor, Department of Chemistry and Biochemistry
South Dakota State University

Dr. Doug Raynie is an associate research professor in the department of chemistry and biochemistry at South Dakota State University. Prior to joining SDSU, he was employed for eleven years as a senior scientist at Procter and Gamble's corporate research division. He earned his PhD at Brigham Young University under the direction of Dr. Milton L. Lee. His undergraduate degree is from Augustana (South Dakota) College, with majors in chemistry and biology. Dr. Raynie’s broad research interests are in the field of sustainability and green chemistry. His two major areas of research are bioprocessing using supercritical fluids and related technologies and analytical separations. Active projects in this area include transformations of lignocellulosic biomass using supercritical fluids and ionic liquids. Analytical separations research includes utilization of deep eutectic solvents, high-resolution chromatography (high-temperature LC and SFC), chromatographic sample preparation (ASE, SFE, SPME, and SPE), chromatography theory, green analytical chemistry, and problem-based learning in analytical chemistry. Dr. Raynie serves on the editorial advisory boards of the Encyclopedia of Separation Science and Pharmaceutical Formulation and Quality.

Tony Taylor
Chief Scientific Officer

Tony comes from a pharmaceutical background and has many years of research and development experience in small- molecule analysis and bioanalysis using LC, GC, and hyphenated MS techniques. Tony is actively involved in method development within the analytical services laboratory at Element Manchester, which supplies contract research in chromatography and mass spectrometry. He continues to research in novel separation technologies, chromatographic method development, and structural characterisation, especially in the areas of extractable and leachable analysis and bio-chromatography. Tony is the technical director of CHROMacademy and has spent the past 22 years as a trainer and developing online education materials in analytical chemistry techniques.

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