The new ultrafleXtremeâ„¢ exceeds all current expectations of MALDI-TOF/TOF technology: A proprietary kHz smartbeam-IIâ„¢ MALDI laser integrated with a novel FlashDetectorâ„¢ and re-engineerd electronics makes it the only MALDI-TOF/TOF on the market to provide kHz acquisition in MS and MS-MS modes. It generates a new level of data quality in applications such as LC-MALDI proteomics, high resolution tissue imaging based biomarker discovery or Top-Down Sequencing.
Ralf Schäfer, Bruker Daltonics Inc.
The new ultrafleXtreme™ exceeds all current expectations of MALDI-TOF/TOF technology: A proprietary kHz smartbeam-II™ MALDI laser integrated with a novel FlashDetector™ and re-engineerd electronics makes it the only MALDI-TOF/TOF on the market to provide kHz acquisition in MS and MS-MS modes. It generates a new level of data quality in applications such as LC-MALDI proteomics, high resolution tissue imaging based biomarker discovery or Top-Down Sequencing.
Advanced electronic engineering and a unique FlashDetector combined with 4 GHz digitization rate at full spectra length and "panoramic" PAN™ technology provide the highest resolving power >40,000 across the peptide mass range (Figure 1) and beyond (Figure 2). This leads directly to increased numbers of identified proteins in LC-MALDI applications at improved specificity.
The proprietary smartbeam-II laser with 1000 Hz repetition rate was specifically developed for the ultrafleXtreme MALDI-TOF/TOF. The software allows operation from 1 to 1000 Hz repetition frequency in MS as well as in MS-MS mode. Therefore, kHz data acquisition of high resolution tissue protein images and LC-MALDI analysis in Biomarker workflows are both perfectly sustained on the ultrafleXtreme. Compared to former instruments a full tissue analysis nowadays can be performed within the same data acquisition time (Figure 3).
In addition, the optimized smartbeam-II laser delivers constant high output energy, regardless of the selected repetition frequency across long acquisition runs. This is indispensable for a series of MALDI imaging runs or LC-MALDI runs.
A computer-controlled variable laser focus diameter of 10–100 μm allows new level spatial resolution — essential for emerging applications like high resolution MALDI tissue imaging. Achieve incredible spatial resolution with no loss of protein signal intensity.
Entirely new design for the removal of sample and matrix debris. Its push-button, laser-cleaning concept is indispensible for kHz operation — and it works for all MALDI matrices. The self-cleaning ion optics is ready for the next run within a few min.
The combination of technologies implemented results in unmatched performance and productivity from the ultrafleXtreme MALDI-TOF/TOF:
The fusion of ultrafleXtreme's novel features shatters existing technological boundaries. The most flexible MALDI-TOF/TOF platform ever demonstrates its ability to be the reference class in MALDI-based Proteomics applications.
(1) Bruker Technical Note TN-15.
(2) A. Holle,A. Haase, M. Kayser & J. Höhndorf, Optimizing UV laser focus profiles for improved MALDI performance (2006) J. Mass Spectrom. 41: 705–716.
(3) Bruker Technical Note TN-32.
For research use only. Not for use in diagnostic procedures.
Bruker Daltonics Inc.
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