Field-Flow Fractionation: Virtual Optimization for Versatile Separation Methods
Flow-field flow fractionation (flow-FFF) offers highly versatile separations for the analysis of complex fluids, covering a size range of macromolecules and particles from 1 nm to 10,000 nm. However, flow-FFF is often perceived as a difficult technique to learn because of the multiple parameters available for adjustment. Recent advances in software for simulating flow-FFF overcome this obstacle, enabling the virtual optimization of flow-FFF methods and opening up the power of flow-FFF separations to non-experts. An added benefit is the ability to easily analyze particle size distributions by elution time from first principles.