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© 2022 MJH Life Sciences and Chromatography Online. All rights reserved.
© 2022 MJH Life Sciences™ and Chromatography Online. All rights reserved.
This instalment describes recent advances and best practices in HPLC analysis of intact monoclonal antibodies (mAbs) and their variants and fragments.
The latest approaches to antibody characterization go beyond ion-exchange, size-exclusion, and reversed-phase modes to make use of affinity, hydrophobic interaction, mixed-mode, and HILIC techniques.