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David Thomas

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Articles

Ultra-High Resolution Chromatography Using 500 mm UHPLC Columns for Detection Of Hidden Sample Components

Ultra-High Resolution Chromatography Using 500 mm UHPLC Columns for Detection Of Hidden Sample Components

Frank Steiner;David Thomas;Felicity Kusinitz;Rainer Bauder
April 19th 2012

LC method development for new drug candidates or screening methods for new active structures in botanicals present the same type of challenges: How do I know that I have separated all main components, as well as important, sometimes hidden, impurities? Is my resolution good enough? Am I missing small, co-eluting peaks?

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Figure 1: Seven prioritization strategies in non-target screening (NTS) by chromatography–HRMS, grouped by domain: chemical information (P1–P3), toxicological information (P5–P6), external information (P4), and preprocessing (P2, P7). Each strategy narrows the candidate space based on specific criteria. Combined, they reduce complexity and focus identification on environmentally relevant features.
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HPLC 2025 Preview: Prioritization Strategies in Non-Target Screening of Environmental Samples by Chromatography with High-Resolution Mass Spectrometry
Nathalie Delaunay, Audrey Combès, and Valérie Pichon © Images courtesy of authors.
Published: May 16th 2025 | Updated: May 25th 2025
HPLC 2025 Preview: Functionalized Monoliths as Selective Sample Preparation Materials
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