From HPLC to UHPLC — And Back
Although ultrahigh-pressure liquid chromatography (UHPLC) method development is fast and cost efficient, many of the analytical methods used in quality control laboratories are still conventional high performance LC (HPLC) separations. Transferring these HPLC methods to UHPLC and validating them is a time-consuming and labour-intensive task. Nevertheless, switching methods from HPLC to UHPLC equipment and vice versa is a powerful tool to increase laboratory efficiency. Modern systems with two flow lines in one system aim to simplify the transition between the two techniques.